乌司他丁对悬浮红细胞储存期凋亡损伤的影响研究

Effects of ulinastatin on storage-associated eryptosis of suspended erythrocytes

目的研究乌司他丁对储存期悬浮红细胞凋亡损伤的影响。方法采集16名健康自愿者血液并按血库常规处理得悬浮红细胞,同一来源的4份悬浮红细胞中分别添加等量生理盐水（对照组）及5,000、10,000、50,000 U/m L的乌司他丁溶液（试验组C1、C2、C3）,在储存0、7、14、21、28及35 d取样检测。使用流式细胞仪检测红细胞体积、磷脂酰丝氨酸（phosphatidylserine,PS）外翻率及细胞内Ca^2＋浓度。结果各组红细胞PS外翻率均自14 d开始升高（P〈0.05）。对照组及C1、C2组红细胞自21d开始明显缩小,而C3组红细胞体积直到28 d才明显缩小,但差异无统计学意义。对照组和C1组细胞内Ca^2＋浓度自35 d开始显著增高（t=16.33,t=14.66,P〈0.05）,而试验组细胞内Ca^2＋浓度均自14 d开始增高;而自21-35 d,C2及C3组细胞内Ca^2＋浓度与对照组相比,差异均无统计学意义。结论在储存期内,悬浮红细胞发生凋亡损伤并随着时间延长而加剧,在储存液里添加适量乌司他丁可在一定程度上抑制凋亡损伤。

Objective To investigate influence of ulinastation in storage period on apoptosis of suspended erythrocyte.Methods RBCs were treated with saline （control group） and different doses of ulinastatin （5,000, 10,000 and 50,000 U/mL in group C1, C2 and C3, respectively）.samples were detected when stored at 0,7,14,21,28,35 d,respectively.Indicators of corpuscular volume,phosphatidylserine extroversion rate and intracellular Ca^2 ＋concentration were analyzed by flow cytometer.Results The phosphatidylserine （PS）-exposure levels of 4 groups started to increase on 14 day（P〈0.05）. Cells of the control group, group C1 and C2 began to shrink remarkably on day 21, while that of Group C3 on 28 day.The intracellular Ca^2 ＋levels of the control group and group C1 started to increase significantly on day 35, （t=16.33,t=14.66,P〈0.05）.one Ca^2 ＋levels of group C1,C2 and C3 increased on day 14.From 21 to 35 day, the intracellular Ca^2 ＋levels of group C2 and C3 were no significant compared with control group.Conclusion During the storage period, suspended erythrocyteapoptosis increase with time prolonged, adding suitable amount of ulinastatin in stock solution can inhibit apoptosis in damage at some level.