利用ITS技术研究苹果砧木遗传多样性

Using ITS to research the genetic diversity of apple rootstocks

目的探索苹果属植物野生砧木间的亲缘关系与杂交亲和性间的联系。方法对24个来自中国不同地域的野生苹果属砧木样品使用nr DNA ITS序列分析,并结合对苹果属植物砧木杂交亲和性的研究。结果本研究成功测出24个样本的序列并做出遗传距离矩阵图与遗传树状图。结论苹果属植物野生砧木间的亲缘关系和砧木杂交亲和性之间有一定的联系。

Objective To explore the relationship between the genetic relationship and the cross compatibility of the wild rootstocks which belong to malus mill plants.Methods With the antecedent study in the cross compatibility of the rootstocks of malus mill plants, this paper focuses on the nrDNA ITS sequence analysis in the samples of the wild rootstocks which belong to malus mill plants from different 24 regions of China.Results It was successfully that having measured the sequence of 24 samples and having made the genetic distance matrix and genetic tree diagram.Conclusion There is a certain relationship between the genetic relationship and the cross compatibility of the wild rootstocks which belong to malus mill plants.