美国山核桃种质资源遗传多样性ISSR分析

Genetic diversity and identification of pecan by ISSR markers

以161个美国山核桃品种及株系、1个核桃品种和1个山核桃品种为试材，利用ISSR分子标记技术对其进行遗传多样性分析。结果表明：14条ISSR引物在供试品种中共扩增出56条谱带，其中55条为多态性条带，多态性指数达98％；供试品种的基因多样性指数为0．3668，Shannon多态性信息指数为0．5428；品种间相似系数范围为0．29-0．94，平均为0．6496，表明这些美国山核桃种质资源间的亲缘关系较近。供试品种（单株）的遗传背景比较复杂，根据聚类分析可将这些品种（株系）划分为9个类群。

In this study, ISSR markers were used to evaluate the genetic diversity of 161 pecan cultivars and individuals, one walnut cultivar and one Carya cathayensis cultivars. A total of 56 bands were yielded by 14 ISSR premiers, and 55 were amplified bands （98% polymorphic）. Nei′s gene diversity He and Shannon′s information index I were 0.366 8 and 0.542 8, respectively. The Nei′s genetic similarity coefficients ranged from 0. 29 to 0. 94, and the average was 0.649 6, which showed close genetic relationships. According to the results of cluster analysis, the 163 accessions could be divided into 9 groups, which showed that the genetic background was very complex.