mir-181c抑制神经母细胞瘤的作用机制研究

mir-181C modulates the proliferation,migration and invasion of neuroblastoma cell

目的研究mir-181c在神经母细胞瘤(NB)中的调控机制。方法采取实时PCR(RT-PCR)、细胞存活实验(MTT)、集落形成试验、Transwell试验。结果 mir-181c下调在原发NB组织比在转移NB组织中更显著。功能性研究显示mir-181c超表达抑制NB细胞增殖、迁移和侵袭。结论 mir-181c在NB中表达下调,mir-181c高表达抑制NB细胞生长,转移和侵袭。

Objective To explore the mir-181 cregulation mechanisms in neuroblastoma（NB）cells.Methods Real-time quantitative PCR（RT-PCR）,cell survival assay（MTT）,cell colony test,Transwell formation test were used in this study.Results We found that mir-181 cwas down-regulated in metastatic NB tissues compared with primary NB tissues.Then functional studies indicated that mir-181cover-expression inhibited NB cell proliferation,migration and invasion,while mir-181 cinhibition increased cell proliferation,migration and invasion.Conclusion mir-181cdown-regulates in NB.High expression of mir-181 cinhibits the growth of NB cell,metastasis and invasion.