摘要
目的观察和分析10只鸵鸟眼球栉膜的解剖学和组织学结构。方法采用常规石蜡切片,苏木素-伊红(HE)染色、Gordon-Sweet法、Van Gieson苦味酸酸性复红染色法,对健康的1 0月龄非洲黑颈鸵鸟眼球栉膜的形态学结构进行观察和分析。结果鸵鸟眼球栉膜位于视乳头部,呈椭圆锥体形浸入玻璃体内。体视显微镜下可见栉膜由三级栉片构成,位于中央的主栉片和主栉片延伸的次级栉片,以及少数次级栉片延伸出的三级栉片。有1个主栉片,次级栉片18~22个,少数次级栉片可以延伸出2~3个三级栉片。栉膜主要由疏松结缔组织构成,含有丰富的胶原纤维、血管及大量黑色素颗粒。结论鸵鸟眼球栉膜的解剖学和组织学结构是研究哺乳动物和人的视网膜血管功能的理想模型。
Objective To investigate the anatomy and histology of 10 ostrichs' pecten in order to provide morphological base for research of ostrich pecten oculi. Methods The morphology of the pecten oculi was observed with a microscope in the 10-month-old healthy Africa black-neck ostrich( Struthio camelus) by hematoxylin-eosin staining,Gordon-Sweet staining,and Van Gieson staining. Results Gross-anatomically,the pecten oculi was attached to the optic disc,and had an irregular cone shape and projected into the vitreous body. In the steromicroscope investigation,the pecten oculi was composed of 1 primary lamella,18-22 secondary lamellae and a few tertiary lamellae. The primary lamella was vertically located in the central part of the pecten,the secondary lamellae were peripherally originated from the primary lamella with the tertiary lamellae which arose from the distal part of some secondary lamella. The pecten consisted of loose connective tissue and was rich in collagen fibers,blood vessel and pigment. Conclusion Pecten oculi in the ostrich is an ideal model for the research of the function of retinal blood vessel form mammal and human.
出处
《解剖学报》
CAS
CSCD
北大核心
2015年第4期450-454,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30800812)
关键词
栉膜
组织切片
苏木素-伊红染色
非洲鸵鸟
Pecten oculi
Tissue sections
Hematoxylin and eosin staining
African ostrich(Struthio camelus)