小鼠精子发生过程中dysbindin-1的表达

Expression of dysbindin-1 during spermatogenesis

目的探讨精子发生过程中dysbindin-1的表达及dysbindin-1对精子顶体形态的影响。方法取生后7d、14d、21d、28d、35d和3月龄的雄性小鼠各3只,用免疫印迹法检测睾丸组织dysbindin-1的表达;以dysbindin-1缺失突变的sdy小鼠为研究对象,收集附睾尾部精子,一部分制备精子涂片,HE染色显示精子形态,用异硫氰酸荧光素-豌豆凝集素(FITC-PSA)和抗精子蛋白56(sp56)单克隆抗体进行荧光染色显示顶体的结构;另一部分采用免疫印迹法检测精子中dysbindin-1的表达。结果不同发育阶段小鼠睾丸组织及精子中均只有dysbindin-1A,无dysbindin-1C的表达;sdy小鼠的精子及顶体的形态未见明显异常。结论 Dysbindin-1A表达于小鼠精子发生的不同时期,dysbindin-1A对精子形态维持不起关键作用。

Objective To test dysbindin-1 expression during spermatogenesis and dysbindin-1 effect on histology of acrosome in mice. Methods Western blotting analysis was applied to detect the expression of dysbindin-1 in testis on day 7,14,21,28 and 35 postpartum and three-month old mice（ n = 3）. The caudal spermatozoa was collected from sdy mice（ no dysbindin-1expression）,HE staining was performed for sperm morphology,fluorescein isothiocyanate conjugate-pisum sativum agglutinin（ FITC-PSA） staining and immunofluorescence staining with antibody against sperm protein 56（ sp56） for acrosome on sperm smear. Results Dysbindin-1A, not-1C, was expressed in postnatal testis development and spermatozoa. Mature sperm from sdy caudal epididymis had normal formation. Conclusion Dysbindin-1A,expressed in testis tissue and sperm during spermatogenesis,is not required for maintaining the morphology of sperm.