JAK/STAT通路介导远距缺血预适应的心肌保护作用

Role of JAK / STAT pathway in cardioprotection of remote ischemic preconditioning

目的:观察远距缺血预适应对大鼠心肌缺血再灌注损伤的心肌保护作用及其机制。方法:24只雄性SD大鼠随机分4组,缺血再灌注(I/R)组,远距缺血预适应(RIPC)组,远距缺血预适应+缺血再灌注(RIPC+I/R)组,远距缺血预适应+AG490+缺血再灌注(AG490)组。取大鼠血液标本及心肌标本。检测相关心肌酶、心肌梗死面积;电镜、光镜测定心肌细胞变化。结果:与I/R组相比,其他3组心梗面积、心肌酶及心肌细胞超微结构变化明显降低(P<0.05)。AG490组与RIPC组相比,心梗面积、心肌酶及心肌细胞超微结构变化明显升高(P<0.05),而与I/R组相比明显降低(P<0.05)。结论:远距缺血预处理具有显著的心肌保护作用。JAK/STAT信号通路参与远距缺血预处理的心肌保护作用。

Objective To investigate the effect of remote ischemic preconditioning on myocardial infarction and the involved protective mechanisms. Methods Twenty-four male SD rats were randomly divided into four groups： ischemia-reperfusion （I/R） group, rote ischemic preconditioning （RIPC） group, remote ischemic preconditioning ＋ ischemia-reperfusion （RIPC＋I/R） group, and RIPC＋AG490＋I/R group. The blood samples and myocardial specimens were collected and prepared for tests. The related enzymes were detected and the size of myocardial infarction was measured. The cardiac cells were determined by electron microscopy and light microscopy. Results The size of myocardial infarct and myocardial enzymes were significantly reduced in RIPC＋I/R group compared to those in I/R group （P 〈 0.05）. The size of myocardial infarction and myocardial enzymes were significantly increased in AG490 group compared to those in RIPC＋I/R group （P 〈 0.05）, but were significantly reduced in AG490 group compared to those in I/R group （P 〈 0.05）. Conclusions Remote ischemic preconditioning may be effective in cardioprotection. The JAK/STAT pathway is involved in the cardioprotection of remote ischemic preconditioning.