卵巢上皮性癌中血管内皮生长因子-C和酪氨酸激酶受体B表达与微淋巴管密度的关系

The Correlation and Significance of VEGF-C and TrkB Protein Expression in Epithelial Ovarian Carcinoma

目的探讨血管内皮生长因子-C(VEGFC)、失巢凋亡抑制因子酪氨酸激酶受体B(Trk B)在卵巢上皮性癌淋巴管形成和淋巴结转移中的作用、与临床病理生物学行为间的关系及两蛋白表达的相关性。方法采用免疫组织化学检测106例原发性卵巢上皮性癌、40例交界性上皮性肿瘤及40例良性上皮性肿瘤组织中VEGF-C和Trk B蛋白的表达,并测定肿瘤组织中微淋巴管密度。结果 VEGF-C在卵巢癌中的表达率为82.1%(87/106),明显高于良性肿瘤组35.0%(14/40)和交界性肿瘤组52.5%(21/40),差异有统计学意义(P<0.05);Trk B在卵巢癌中的表达率为74.5%(79/106),明显高于良性肿瘤组7.5%(3/40)和交界性肿瘤组27.5%(11/40),差异有统计学意义(P<0.05);D2-40标记的瘤内淋巴管密度(MLVD)在卵巢癌组为(14.25±3.57),明显高于良性肿瘤组(8.87±4.46)和交界性肿瘤组(11.74±5.32)(P<0.05)。在卵巢癌组中,VEGF-C和Trk B蛋白的表达呈正相关(r=0.574,P<0.05),VEGF-C、Trk B的表达与瘤组织内MLVD数呈正相关(r分别为0.488,0.510,P<0.05)。在肿瘤分化越低、临床分期越高、脉管内有癌栓及有淋巴结转移的卵巢癌组织中,Trk B和VEGF-C蛋白的阳性率越高,MLVD数越多(P<0.05)。结论 VEGF-C可能与Trk B在卵巢癌的发生发展过程中发挥着协调作用,促进卵巢癌中淋巴管形成及淋巴结的转移,联合检测VEGF-C、Trk B及MLVD可作为判断卵巢癌恶性程度、侵袭、预后的重要指标。

Objective To evaluate the correlation of VEGF-C and Trk B protein expression and explore its role in the in epithelial ovarian carcinoma lymphangiogenesis and lymph node metastasis. Methods VEGF-C and Trk B protein were detected in106 cases of epithelial ovarian carcinoma,40 cases of borderline tumors and 40 cases of benign tumors by immunohist-ochemistry and MLVD was detected. Results In the ovarian carcinoma,the positive expression rate of VEGF-C protein was 82. 1%. Significantly higher than in benign tumors（ 35. 0%） and borderline tumors（ 52. 5%,P〈0. 05）. In epithelial ovarian carcinoma,the positive expression rate of Trk B was 74. 5%. signifcantly higher than in benign tumors（ 7. 5%） and borderline tumors（ 27. 5%,P〈0. 05）. The MLVD in ovarian carcinoma was（ 14. 25±3. 57）,which was higher than those in benign tumors（ 8. 87±4. 46） and borderline tumors（ 11. 74±5. 32）,P〈0. 05）. And in ovarian carcinoma,the VEGF-C expression was positively correlated with the Trk B（ r = 0. 574,P〈0. 05）. There ware positive relationship between the expression of VEGF-C,Trk B and MLVD in ovarian carcinoma（ r = 0. 488,0. 510,P〈0. 05）. In poor differentiation,high clinical stage,lymph node metastasis and in the vascular invasion group,the high expression rate of VEGF-C,Trk B and the high MLVD were observed（ P〈0. 05）. Conclusion The high expression of VEGF-C and Trk B may exhibit synergistic effects on the development of ovarian carcinoma and simultaneously promote malignant development of ovarian carcinoma. The combined detection of two proteins can be nseds as inportant indicators to determine the degree of malignancy of ovarian cancer.