腰背痛患者520例人类白细胞抗原-B27检测及亚型分型

Determination of HLA-B27 and its subtypes in 520 patients with low back pain

目的探讨人类白细胞抗原B27(HLA-B27)的检测及其亚型分型。方法采用流式细胞术(FCM)及聚合酶链反应-序列特异性引物(PCR-SSP)法对520例腰背痛患者外周血HLA-B27及其亚型进行检测。结果在520例腰背痛患者中,FCM阳性率为55.4%,PCR-SSP法阳性率为56.7%。两者的阳性率差异无统计学意义(χ2≈3.79,P>0.05)。PCR-SSP法检出HLA-B2704、HLA-B2705、HLA-B2705/07、HLA-B2702、HLA-B2704/05、HLA-B2705/02、HLA-B2704/02、HLA-B2704/05/07等亚型,分别占42.0%、29.2%、23.1%、1.4%、0.7%、0.7%、0.3%。结论 FCM与PCR-SSP法是目前常用的检测HLA-B27的方法,两者阳性率差异无统计学意义,但PCR法可进行亚型分型,这些亚型与强直性脊柱炎等疾病的诊断、治疗及预后判断相关。

Objective To investigate the determination of human leukocyte antigen-B27（HLA-B27） and its subtypes. Methods Flow cytometry method（FCM） and polymerase chain reaction-sequence specific primer（PCRSSP） were used to determine the expression of HLA-B27 and its subtypes in peripheral blood of 520 patients with low back pain. Results In the 520 patients with low back pain, the positive rate of FCM and PCR-SSP was 55.4% and56.7%, respectively. The chi-square test showed that χ2≈3.79, P〉0.05, without statistically significant difference in the positive rate between FCM and PCR-SSP. The subtypes of HLA-B2704, HLA-B2705, HLA-B2705/07, HLAB2702, HLA-B2704/05, HLA-B2705/02, HLA-B2704/02, and HLA-B2704/05/07 accounted for 42.0%, 29.2%, 23.1%,1.4%, 0.7%, 0.7%, 0.3%, respectively. Conclusion FCM and PCR-SSP are the common methods to determine HLAB27, without significant difference in positive rate between them. However, PCR-SSP can classify the subtypes, which are associated with the clinical diagnosis, treatment and prognosis of diseases such as ankylosing spondylitis.