谷氨酰胺对慢性阻塞性肺疾病患者外周血IL-6及HSP70表达的影响

目的：观察谷氨酰胺（Gin）对慢性阻塞性肺疾病（COPD）患者外周血单个核细胞（PBMC）中IL-6和热休克蛋白70（HSP70）表达的影响。方法：收集COPD急性加重期（AECOPD）、COPD稳定期（SCOPD）各30例外周血PBMC，将AECOPD、SCOPD患者外周血PBMC各分为Gin组和空白对照组两组，健康组外周血PBMCl5例；应用Real—timePCR法测定五组外周血PBMC中IL-6、HSP70的基因表达。结果：健康对照组较AECOPD和SCOPD空白对照组IL-6、HSP70的表达低，差异有统计学意义（均P〈0．05或P〈001）；AECOPD纽和SCOPD组中，用Gin干预的较空白对照组PBMC中HSP70的表达均升高，差异有统计学意义（均P〈0．05），而IL-6表达均下降（均P〈0．05）。结论：Gln可抑制COPD患者炎症因子IL-6的活性，升高HSP70的表达。

Objective： To investigate the effects of glutamine on the expression of IL - 6 and HSP70 in PBMC of patients with COPD . Methods ： PBMCs were collected from COPD patients with acute exacerbation （AECOPD group, n = 30） , stable COPD patients （SCOPI） group, n = 30） and 15 health controls. Two COPD groups were further divided into 4 subgroups ： blank control and glutamine （Gin） groups. The levels of IL - 6 and HSP70 gene expression in PBMCs were detected by real - time PCR. Results ：The mRNA expression of IL - 6 and HSP70 in blank control group of AECOPD and SCOPD were higher than those in the normal group （ P 〈 0. 05 or P 〈 0. 01 ）. After treated by glutamine,the expression of HSP70 increased in both AECOPD and SCOPD patients （ P 〈 0. 05 ）, while the expression of IL - 6 decreased （P 〈 0. 05 ）. Conclusion ： Glutamines could inhibit mRNA expression of IL -6 and increase HSP70.