长柄扁桃茎尖离体分化成苗的培养基筛选

Media Screening for Stem Tip Differentiation into Sprout

探索河北丰宁、包头固阳、内蒙乌审旗和陕西神木等四产地长柄扁桃茎尖离体分化成苗的最适培养基,为优良品种的种质资源保存和大量繁殖奠定基础。以MS、WPM和B5培养基为基础培养基,添加不同浓度的生长素和细胞分裂素,共组成48种培养基,每种培养基分别接种长柄扁桃优良品种茎尖50个,茎尖大小为0.3～0.5mm,25℃、16h/3 000Lx光照培养,20℃、8h暗培养,10d后统计茎尖离体分化成苗情况。实验结果得出,10d后形成黄绿色的愈伤组织,25～30d时可分化出幼苗,培养基为WPM＋NAA0.1mg/L＋BAP1.0mg/L＋GA3 0.10mg/L时,四产地长柄扁桃最适合茎尖分化成苗率均最高,河北丰宁、内蒙乌审旗、包头固阳和陕西神木四产地的分别为62%、54%、48%和60%,且幼苗生长健壮。WPM培养基是长柄扁桃茎尖离体培养最合适的基础培养基,在筛选激素组合的培养基上培养可获得大量的长柄扁桃组培苗。

Experiments were conducted to find out the proper media for stem-tip differentiation into sprout of Prunes pedunculata collected from Fengning of Hebei, Guyang of Baotou, Wushengqi and Shenmu of Shaanxi. MS, WPM and B5 were used as media respectively, added with different concen- trations of auxin and cytokinin. 48 different media were formed to cultivate 50 stem--tip sized 0.3～ 0.5 mm for illumination incubation with 16 h/3000 Lx at temperature 25℃or dark incubation at tem- perature 20℃ for 8h. After 10d, the differentiation of stem-tip was calculated. The result showed that after 10 d, yellow-green callus were formed and after 25～30 d the differentiation into seedlings were formed. When media WPM-I-NAA0. lmg/L-I-BAP1.0 mg/L -kGA3 0. 10 mg/L was applied, differentiation of stem-tips of Prunes pedunculata from four locations produced maximum survival seedlings and survival rate were 62 %. 54 %. 48 % and 60 %, respectively. WPM is proved to be the best basic medium for stem-tip differentiation of Prunes pedunculata.