产ESBLs肺炎克雷伯菌耐药基因研究及其基因分型

ESBLs producing bacteria klebsiella drug resistance gene and gene typing

目的：对临床分离产ESBLs肺炎克雷伯菌相关基因进行研究，并对其进行基因分型。方法：临床分离295株肺炎克雷伯菌，ESBLs药敏试验采用K-B法。PCR法检测I类整合酶、I类整合子基因盒、ISCR1、blaTEM、blaSHV、blaCTX-M。I类整合子PCR扩增阳性产物送去测序，测序结果在GenBank中用blastn进行核酸序列同源性搜索。菌株基因分型采用ERIC-PCR法。结果：产ESBLs肺炎克雷伯菌阳性率为21.3%。在63株产ESBLs肺炎克雷伯菌中，分为35个基因型，I类整合酶、ISCR1、blaTEM、blaSHV、blaCTX-M的阳性率分别为46.0%、12.7%、55.6%、0、81.0%，27个I类整合子含有7种类型基因盒。结论：在产ESBLs肺炎克雷伯菌中，I类整合子携带率较高，ESBLs基因型以blaTEM和blaCTX-M为主。我院产ESBLs肺炎克雷伯菌存在某种流行株。

Objective：Of clinical separation ESBLs pneumonia klebsiella bacteria related genes, and genes.Methods：Clinical isolated 295 strains of pneumonia klebsiella bacteria, ESBLs drug sensitive test using the K - B method. PCR method to detect the I class integrase, class I integron gene box, ISCR1, blaTEM, blaSHV, blaCTX -m. Class I integron PCR amplification positive product sent to sequencing, the sequencing results in using blastn GenBank nucleic acid sequence homology search. Strain genotyping by ERIC - PCR method.Results： Producing ESBLs pneumonia klebsiella bacteria positive rate was 21.3%. In 63 strains producing ESBLs pneumonia klebsiella bacteria, divided into 35 genotypes, I class integrase, ISCR1, blaTEM, blaSHV, blaCTX - M positive rate were 46.0%, 12.7%, 55.6%, 0, 81.0%, 27 class I integron box containing the 7 types of gene.Conclusions：In producing ESBLs pneumonia klebsiella bacteria, class I integron carried rate is higher, ESBLs genotypes is given priority to with blaTEM and blaCTX - M. Our producing ESBLs pneumonia klebsiella bacteria exist some kind of epidemic strains.