丙泊酚预处理对人胃黏膜上皮细胞缺氧-复氧损伤的保护作用

Protective effect of propofol on hypoxia-reoxygenation injury in human gastric epithelial cells

目的研究丙泊酚预处理对人胃黏膜上皮GES-1细胞缺氧-复氧(H-R)损伤的影响。方法将GES-1细胞随机分为六组:N组为正常对照;其余五组采用缺氧1.5h,复氧2h建立H-R模型。缺氧前加入无糖培养基RPMI-1640,并分别行药物预处理:P组加入丙泊酚50μmol/L,F组加入脂肪乳,E组加入表没食子儿茶素没食子酸酯,E+P组加入表没食子儿茶素没食子酸酯和丙泊酚;H-R组为模型对照。采用MTT法检测细胞活力,荧光染色法检测细胞凋亡,实时定量PCR和Western blot检测Toll样受体4(TLR4)mRNA和蛋白表达。结果丙泊酚预处理能增强H-R条件下GES-1细胞的活力(P<0.01),减少细胞凋亡(P<0.01),降低GES-1细胞的TLR4mRNA和蛋白表达(P<0.01)。结论丙泊酚预处理可减轻GES-1细胞的H-R损伤,其保护作用可能与降低TLR4表达有关。

Objective To investigate the effect of propofol on hypoxia-reoxygenation（H-R）injury in human gastric epithelial（GES-1）cells.Methods The cultured GES-1cells were randomly assigned into six groups.Group N was taken as blank control.The H-R models were established by hypoxia for 90 minutes and reoxygenation for 120 minutes.Before hypoxia,the culture medium was replaced by glucose-free RPMI-1640,into which propofol 50μmol/L（group P）,fat emulsion（group F）,epigallocatechin gallate（group E）,or epigallocatechin gallate and propofol（group EP）was added as the pretreatment.Cell viability was detected by MTT assay and apoptosis was determined by Hoechst 33258 fluorochrome staining.The expressions of Toll-like receptor 4（TLR4）mRNA and protein were detected by quantitive real time PCR and Western blot,respectively.Results Pretreated with propofol 50μmol/L could markedly enhance the viability and decrease the apoptosis of GES-1cells under H-R injury（P〈0.01）.Propofol preconditioning could decrease the expressions of TLR4 mRNA and protein in GES-1cells under H-R injury（P〈0.01）.Conclusion Propofol preconditioning may relieve H-R injury of GES-1cells,which may be related with a decrease of TLR4 expression.