新辅助化疗对乳腺癌细胞周期及干细胞的影响

Effect of neoadjuvant chemotherapy on breast cancer cell cycle and breast cancer stem cells

目的探讨新辅助化疗对乳腺癌患者细胞周期中G0-G1期细胞比例以及三磷酸腺苷结合转运蛋白G超家族成员2（ABCG2）、CD44^＋CD24^-/low表达的影响。方法选取2013年5月至2014年3月收治的60例经空芯针穿刺活检明确病理诊断的初治乳腺浸润性导管癌患者为研究对象，比较化疗前后乳腺癌细胞周期中G0-G1期细胞比例及ABCG2、CD44^＋CD24^-/low含量的变化。结果患者化疗后ABCG2为（25.10±1.50）%，CD44^＋CD24^-/low为（36.40±3.80）/10^5，G0-G1期细胞比例为（70.50±1.50）%，均高于化疗前的（15.88±1.22）%、（25.00±3.40）/10^5、（60.65±1.30）%，差异具有统计学意义（t=8.685，P＜0.05；t=9.226，P＜0.05；t=8.898，P＜0.05）。所有患者完成4个疗程的化疗后，cCR率、cPR率、SD率分别为18.3%（11/60）、73.3%（44/60）、8.3%（5/60）。结论新辅助化疗后患者肿瘤细胞发生周期阻滞，肿瘤干细胞比例上升，疗效确切，具有临床推广价值。

ObjectiveTo investigate the effect of neoadjuvant chemotherapy on the proportion of G0-G1 phase cells and the expressions of ATPbinding cassette subfamily G member 2 （ABCG2） and CD44^＋CD24^-/low in breast cancer patients. MethodsSixty untreated cases with breast invasive ductal carcinoma from May 2013 to March 2014 were chosen. All patients were tested by core needle biopsy and pathological diagnosis, then treated by neoadjuvant chemotherapy. The proportion of G0-G1 phase cells and the contents of ABCG2 and CD44^＋CD24^-/low before and after therapy were compared. ResultsAfter chemotherapy, the contents of ABCG2 and CD44^＋CD24^-/low were （25.10±1.50）% and （36.40±3.80）/10^5, and the proportion of G0-G1 phase cells was （70.50±1.50）%, which were higher than those before treatment [（15.88±1.22）%, （25.00±3.40）/10^5, （60.65±1.30）%] （t=8.685, P〈0.05; t=9.226, P〈0.05; t=8.898, P〈0.05）. All patients completed four courses of chemotherapy, and the cCR rate, cPR rate, SD rate were respectively 18.3% （11/60）, 73.3% （44/60）, 8.3%（5/60）. ConclusionCell cycle can be arrested and the proportion of stem cells can be raised after neoadjuvant chemotherapy which has exact curative effect and clinical popularization value.