摘要
以无花果为组织培养试验材料,采用改变培养条件和使用不同培养基的方法,研究培养条件对外植体褐化的影响。结果表明:无花果外植体褐化在WPM培养基上比MS培养基上较轻,培养基中琼脂为9g的无花果外植体褐化较轻,经过暗处理的无花果外植体褐化程度较轻,以及低温条件能有效缓解外植体褐化。
Taking Ficus carica L.as a tissue culture experiment material,adopting the method of changing culture conditions and using different culture medium,the effect of culture conditions on the explants browning was studied.The results showed that on WPM medium the explant browning was lighter than on MS medium;the explant browning was lighter on 9g agar medium and under dark treatment;low temperature could effectively alleviate the explant browning.
出处
《北方园艺》
CAS
北大核心
2015年第14期99-101,共3页
Northern Horticulture
基金
湖北第二师范学院"植物抗癌活性物质提纯与应用湖北省重点实验室"建设资助项目
关键词
无花果
组织培养
褐化
Ficus carica L.
tissue culture
browing
