颠茄植株再生体系的建立

Establishment of Regeneration System of Atropa belladonna

以颠茄种子萌发的无菌苗为试材,采用组织培养方法,以MS为基本培养基,研究IAA、NAA、6-BA、KT对愈伤组织诱导及分化的影响,探索适宜颠茄愈伤组织诱导和分化的培养基。结果表明：在MS＋KT 0.5mg/L＋6-BA 1.0mg/L和MS＋KT 1.0mg/L＋6-BA 0.5mg/L培养基上,愈伤诱导率较高达100%,KT、6-BA添加生长素配比诱导愈伤组织效果不佳。KT单独诱导的愈伤组织宜在20d后转接,KT和6-BA配合使用诱导出的愈伤组织宜在40d后转接。生根适宜培养基为1/2MS＋NAA 0.2-0.4mg/L。该研究建立了颠茄的植株再生体系,为颠茄的组织培养提供了技术体系。

Taking seedlings of Atropa belladonnaas explants,by tissue culture methods,MS as basic culture medium with different concentrations of IAA,NAA,6-BA and KT were used to establish the optimal media for callus induction and differentiation.The results showed that 100%of calli induction rate was obtained on MS medium with KT 0.5mg/L＋6-BA 1.0mg/L and KT 1.0mg/L＋6-BA 0.5mg/L.Calli could not be induced on MS medium with the combination of auxin and KT or 6-BA.The induced calli on MS media with KT or KT with 6-BA were subcultured optimally at 20 days and 40 days.1/2MS＋NAA 0.2-0.4mg/L was the optimal medium for plants＇ rooting.This study would provide the technological system of tissue culture for Atropa belladonnaregeneration.