单叶蔓荆果实中多甲氧基黄酮类成分的分离、鉴定及细胞毒活性分析

Isolation,identification and cytotoxic activity analysis of polymethoxylated flavonoids from Vitex rotundifolia fruit

单叶蔓荆（Vitex rotundifolia Linn.f.）为马鞭草科（Verbenaceae）牡荆属（Vitex Linn.）多年生落叶灌木,其干燥成熟果实供药用,具有疏散风热的功效[1]。单叶蔓荆生于沙滩、海边及湖畔,主要产于中国安徽、福建、广东、河北、江苏、江西、辽宁、山东、台湾和浙江等地,日本、东南亚地区和太平洋群岛也有分布[2]。

Six polymethoxylated flavonoids were isolated from ethyl acetate fraction of ethanol extracts from Vitex rotundifolia Linn. f. fruit, and their structures were identified by NMR and MS methods, and cytotoxic activity of six compounds are analyzed by MTT method. The six compounds are oroxylin A （ 1 ） , chrysosplenol-D （ 2 ） , casticin （ 3 ） , artemetin （4）, persieogenin （ 5 ） and 5,3＇-dihydroxy-6,7,4＇-trimethoxyflavanone （6）. In which, persicogenin is isolated from V. rotundifolia for the first time. Oroxylin A, casticin, artemetin and 5,3＇-dihydroxy-6,7,4＇-trimethoxyflavanone can inhibit proliferation of human liver cancer cell line HepG2 in vitro, and their IC5o is 11.90, 12.54, 13.69 and 11.76 μmol·L^-1, respectively, showing that the four compounds have a certain cytotoxic activity.