2,4-滴丁酯对雄性小鼠睾丸的氧化损伤及血清睾酮的影响

Testis Oxidative Damage and Serum Testosterone Variations in Male Mice Induced by 2, 4-D Butylate

[目的]探讨2,4-滴丁酯对雄性小鼠睾丸的氧化损伤及血清睾酮的影响。[方法]将48只清洁级健康雄性ICR小鼠按体重随机分为4组,每组12只,分别为对照组(玉米油)和低(10 mg/kg)、中(20 mg/kg)、高剂量(40 mg/kg)2,4-滴丁酯染毒组。采用灌胃的方式进行染毒,1次/d,每周连续染毒6 d,实验周期为5周。染毒结束24 h后,采用放射免疫分析法测定血清中睾酮的含量;取睾丸测定脏器系数及采用分光光度法对睾丸组织中超氧化物歧化酶(SOD)活力和丙二醛(MDA)的含量进行测定。[结果]染毒4周时,与对照组比较,中剂量组和高剂量组小鼠体重增长幅度明显降低,差异有统计学意义(P<0.05);染毒5周时,与对照组和低剂量组相比,中剂量组与高剂量组小鼠体重增长幅度明显降低,差异有统计学意义(P<0.05);小鼠睾丸脏器系数随染毒浓度的增大呈下降趋势,与对照组和低剂量组相比,高剂量组睾丸脏器系数值较低,且差异有统计学意义(P<0.05);SOD活力随着2,4-滴丁酯染毒剂量的升高逐渐降低,各实验组间差异均有统计学意义(P<0.05);MDA含量随着2,4-滴丁酯染毒剂量的升高逐渐增高,高剂量组与对照组之间的差异有统计学意义(P<0.05);血清中睾酮水平随着染毒剂量的升高逐渐降低,与对照组和低剂量组比较,高、中剂量组血清中睾酮水平较低,差异有统计学意义(P<0.05),且高剂量组与中剂量组之间差异有统计学意义(P<0.05)。[结论]2,4-滴丁酯可导致雄性小鼠睾丸组织氧化损伤并降低血清中睾酮水平。

[Objective] To explore the effects of 2, 4-D butylate on testis oxidative damage and serum testosterone in male mice. [Methods] Forty-eight healthy clean-grade ICR male mice were randomly divided into control（corn oil）, low, middle, and high dose groups（10, 20, 40 mg/kg） by weight, twelve in each group. Oral gavage administration was conducted once a day and six days per week for consecutive five weeks. After 24 h of exposure, testosterone levels in serum was detected by radioimmunoassay; testis was weighed to calculate organ coefficient, and the activity of superoxide dismutase（SOD） and the content of malondialdehyde（MDA） in testicular homogenate were measured by spectrophotometric assay. [Results] After four weeks, the increased body weight was significantly decreased in the middle dose and the high dose groups compared with the control group（P〈0.05）; after five weeks, the increased body weight was significantly decreased in the middle dose and the high dose group compared with the control and the low dose groups（P〈0.05）. The organ coefficient of testis was gradually decreased as the exposure dose increased, and the difference was significant between the high dose group and the middle dose group or control group（P〈0.05）. The activity of SOD was gradually decreased as the dose increased, and the difference between the experimental groups was statistically significant（P〈0.05）; the content of MDA was gradually increased as the dose increased, and the difference between the high dose group and the control group was statistically significant（P 0.05）; the testosterone levels in serum were gradually decreased as the dose increased, those in the middle and high dose groups were significantly lower than those in the low dose group or the control group（P〈0.05）, and significant difference was found between the high dose and the middle dose groups（P〈0.05）. [Conclusion] The findings indicate that 2, 4-D butylate could lead to oxidative damage in testis and lower testosterone levels in serum of male mice.