血卟啉单甲醚荧光光谱法辅助诊断早期口腔癌

Hematoporphyrinmonomethyl Ether Fluorescence Spectrometry Auxiliary Diagnosis of Early Oral Cancer

目的:应用动物模型探讨血卟啉单甲醚(hematoporphyrinmonomethyl ether,HMME)介导的荧光光谱法辅助诊断早期口腔癌的可行性。方法:21只金黄地鼠随机分成3组(对照组、5周组、9周组),每组7只,各组动物由DMBA诱导双侧颊囊相应时间后,按10ml/kg经腹腔注射20mg/L HMME溶液,用光谱诊断装置分别记录自体及HMME荧光,应用Origin7.0软件计算荧光比值K值,检测位点经病理切片HE染色定性.计算各组K值均值,SPSS10.0统计分析。结果:动物实验结果经方差分析,3组K值均值相比有统计学意义(P<0.01)。结论:HMME介导的荧光光谱法能够区分正常、异常增生和癌变组织,对口腔早期癌的诊断具有重要参考价值。

Objective：To investigate the feasibility of fluorescence spectra diagnosis of early oral cancer mediated with hematoporphyrinmonomethyl ether（HMME）in animal models.Methods：21golden hamsters were randomly divided into 3groups（control group,5weeks group and 9weeks group）.Each group had 7animals.After induced by DMBA in corresponding weeks all the cheek pouches of animal models in each group were injected through intraperitoneal by 20ug/ml HMME solution as 10ml/kg.HMME fluorescence and auto-fluorescence spectrums were detected by fluorescence spectroscopy diagnosis system and were analyzed by Origin7.0to calculate fluorescence ratio K.All the detected points were determined by pathological diagnose.Mean K values were calculated and statistically analyzed by SPSS10.0.Results：The result of animal experiment was analyzed by ANOVA.It showed that the comparisons of K values among 3experiment groups had statistical significance（P〈0.01）.Conclusion：Fluoresscent-spectro fluorometry method mediated by HMME could discriminate normal tissue,abnormal hyperplasia and cancer tissue.It had important reference value in diagnosis of early oral cancer.