摘要
[目的]观察止痛巴布贴对腹膜炎疼痛模型小鼠扭体反应影响。[方法]使用随机平行对照方法,将70只(热板痛阈值7-30S)小鼠按编号随机数字表法分为7组,10只/组。清洁级动物室(恒温16-24℃,湿度450%-55%,日照12h),自由觅食饮水7d。8d起干预,空白组:不予处置;基质组:空白基质贴外用;扶他林组:扶他林软膏250mg/kg外用;止痛巴布贴1、2、3、4组(延胡索、红花、桃仁、马钱子、青风藤等八味):止痛巴布贴1.52g/kg、4.55g/kg、13.65g/kg、27.30g/kg外用;41gd。连续给药7d后(第14d)),醋酸扭体法复制人工腹膜炎疼痛模型,观测扭体反应。[结果]腹腔注射药物15min,各药物干预组小鼠扭体频率明显降低;基质组与空白组无显著差异(P〉0.05);止痛巴布贴l、2、3、4组均低于空白组(P〈0.05,P〈O.01),止痛巴布贴1、2组高于扶他林组(P〈O.01);止痛巴布贴3、4组与扶他林组无显著差异(P〉0.05)。[结论]止痛巴布贴可降低小鼠腹膜炎疼痛模型扭体次数,影响随用药剂量增加递增(量效关系)。
[Objective]To observe the Relieve Pain Plaster effects of Body torsion reaction in experimental mice.[Methods]Dsing random parallel control method,70(7-30s)hot plate pain threshold of mice according to the number of random number table method divided into 7 groups,10/group.Clean level housed separately(constant temperature 16-24 ℃,humidity 45%-55%,rizhao 12h),free feeding water 7d.8d intervention,blank group:no disposal;Matrix groups: blank substrate paste for external use;Give he Lin groups: did he Lin ointment 250 rag/ kg for external use;Analgesic buiter, 1,2,3,4 group(rhizoma corydalis,safflower,peach kernel,nux vomica,orientvine eight taste):analgesic buiter, l.52g/kg,4.55g/kg,13.65g/kg,27.30g/kg for external use;4h/d.Continuous dosing after 7d),acetic acid twisting method artificial peritonitis pain model,observation body torsion reaction.[Results] Intraperitoneal injection of drugs for 15 min,the drug intervention group mice body torsion frequencies decrease;Matrix with the blank group no significant difference(P〉0.05);Analgesic buiter, 1,2,3,4 group were lower than that of blank group(P〈0.05, P〈0.01),and the pain it 1 and 2 above Iifted him Lin group(P 〈0.01);Pain,buiter,3,4,and lifted him Lin group no significant difference(P〉0.05).[Conclusion]Relieve Pain Plaster can decrease the number of peritonitis pain model in mice body torsion,influence increasing with the increase of dosage(concentration-response relationship).
出处
《实用中医内科杂志》
2015年第7期128-130,共3页
Journal of Practical Traditional Chinese Internal Medicine
关键词
腹膜炎疼痛模型小鼠
止痛巴布贴
扶他林
醋酸扭体反应
热板痛阈值
量效关系
随机平行对照研究
peritonitis pain model mice
relieve pain plaster
voltar
acetic acid twisting body response
hot plate pain threshold
oncentration-response relationship
random parallel control study