止痛巴布贴对腹膜炎疼痛模型小鼠扭体反应影响随机平行对照研究

Effects of Relieve Pain Plaster in Body Torsion Reaction of Mice Peritonitis Model Random Parallel Control Study

[目的]观察止痛巴布贴对腹膜炎疼痛模型小鼠扭体反应影响。[方法]使用随机平行对照方法，将70只（热板痛阈值7-30S）小鼠按编号随机数字表法分为7组，10只／组。清洁级动物室（恒温16-24℃，湿度450%-55％，日照12h），自由觅食饮水7d。8d起干预，空白组：不予处置；基质组：空白基质贴外用；扶他林组：扶他林软膏250mg／kg外用；止痛巴布贴1、2、3、4组（延胡索、红花、桃仁、马钱子、青风藤等八味）：止痛巴布贴1．52g／kg、4．55g／kg、13．65g／kg、27．30g／kg外用；41gd。连续给药7d后（第14d）），醋酸扭体法复制人工腹膜炎疼痛模型，观测扭体反应。[结果]腹腔注射药物15min，各药物干预组小鼠扭体频率明显降低；基质组与空白组无显著差异（P〉0．05）；止痛巴布贴l、2、3、4组均低于空白组（P〈0．05，P〈O．01），止痛巴布贴1、2组高于扶他林组（P〈O．01）；止痛巴布贴3、4组与扶他林组无显著差异（P〉0．05）。[结论]止痛巴布贴可降低小鼠腹膜炎疼痛模型扭体次数，影响随用药剂量增加递增（量效关系）。

[Objective]To observe the Relieve Pain Plaster effects of Body torsion reaction in experimental mice.[Methods]Dsing random parallel control method,70（7-30s）hot plate pain threshold of mice according to the number of random number table method divided into 7 groups,10/group.Clean level housed separately（constant temperature 16-24 ℃,humidity 45%-55%,rizhao 12h）,free feeding water 7d.8d intervention,blank group：no disposal;Matrix groups： blank substrate paste for external use;Give he Lin groups： did he Lin ointment 250 rag/ kg for external use;Analgesic buiter, 1,2,3,4 group（rhizoma corydalis,safflower,peach kernel,nux vomica,orientvine eight taste）：analgesic buiter, l.52g/kg,4.55g/kg,13.65g/kg,27.30g/kg for external use;4h/d.Continuous dosing after 7d）,acetic acid twisting method artificial peritonitis pain model,observation body torsion reaction.[Results] Intraperitoneal injection of drugs for 15 min,the drug intervention group mice body torsion frequencies decrease;Matrix with the blank group no significant difference（P〉0.05）;Analgesic buiter, 1,2,3,4 group were lower than that of blank group（P〈0.05, P〈0.01）,and the pain it 1 and 2 above Iifted him Lin group（P 〈0.01）;Pain,buiter,3,4,and lifted him Lin group no significant difference（P〉0.05）.[Conclusion]Relieve Pain Plaster can decrease the number of peritonitis pain model in mice body torsion,influence increasing with the increase of dosage（concentration-response relationship）.