人参皂苷Rb_1改善术后疲劳综合征大鼠中枢炎症反应的机制研究

Improvement of ginsenoside Rb_1 on inflammation of central nervous system in postoperative fatigue syndrome rats

目的研究术后疲劳综介征(POFS)大鼠海马内炎症因子及经典炎症通路变化,探讨POFS的中枢发生机制,并考察人参皂昔Rb,对中枢疲劳的十预作用。方法将96只SD大鼠随机分为对照组、POFS模型组(POFS组)和人参皂昔Rb1,十预组(人参皂昔Rb,组),每组再按时间点分为术后1、3、5、7 d 4个亚组。术后对应时间点进行旷场实验,实时荧光定量PCR检测大鼠海马炎症因子的mRNA表达;Western blotting检测磷酸化p38 (p-p38)、核因子KB/p65 <NF-KB/p65)蛋自表达;免疫组化法检测海马内p-p38蛋自表达;免疫荧光法检测海马内NF-icB/p65蛋自核内外分布。结果术后1、3 d,与对照组比较,POFS组大鼠运动距离减少(P<0.01),休息时间增加(P<0.05);中枢内炎症因子水平升高(P<0.05);p-p38蛋自表达也增加(P<0.05),(NF-KBfp65胞核)/(NF-oBfp65胞浆)增加(P<0.05);人参皂昔Rb1,组与POFS组比较,术后1、5 d,大鼠休息时间明显缩短CPS 0.05;术后1. 3 d,大鼠运动距离增加(P<0.01),炎症因子表达下降(P<0.05)(NF-oB/p65胞核)/(NF-KB/p65胞浆)明显减少(P<0.05);术后3、5、7 d p-p38蛋自表达也降低(P<0.05)。免疫组化、免疫荧光结果与Western blotting结果相同。结论 POFS大鼠海马内炎症因子表达升高,经典炎症通路激活,人参皂昔Rb1,可通过抗炎作用来改善POFS大鼠的中枢疲劳。

Objective To explore the inflammatory factor and inflammation signal pathway in hippocampus of postoperative fatigue syndrome （POFS） rats, and to investigate the central mechanism of POFS and the anti-fatigue effect of ginsenoside Rb1. Methods Ninety-six male SD rats were randomly divided into control, POFS, and ginsengoside Rb1 intervention groups （ginsengoside Rb1 groups）, and each group was divided into subgroups by postoperative 1, 3, 5, and 7 d. The fatigue was assessed with open field test. The mRNA levels of inflammatory cytokines in hippocampus were measured by real-time quantitative PCR. The activation of p38MAPK enzyme was examined by Western blotting and immunohistochemisty. The translocation of NF-κB/p65 in nuclear was measured by Western blotting and immunohistofluorescence. Results On postoperative day 1 and day 3, compared with the control group, the journey of rats in the POFS group declined （P 〈 0.01）, while resting time increased （P 〈 0.01）, the level of inflammation cytokines added, the expression of p-p38MAPK protein was enhanced and the ratio of NF-κB/p65 cytoplasm/NF-κB/p65/nuclear was also elevated （P 〈 0.05）. Compared with the POFS group, the resting time of rats decreased （P 〈 0.05） on postoperative day 1 and day 5.Onday 1 and day 3 after surgery, the journey of rats was enhanced （P 〈 0.01）, the level of inflammation cytokines was declined and the ratio ofNF-κB/p65 cytoplasm/NF-κB/p65/nuclear was decreased （P 〈 0.05）. The levels ofp-p38MAPK in postoperative day 3, day 5, and day 7 were also declined （P 〈 0.05）. The results of immunohistochemisty and immunohistofluorescence were accordance with Western blotting. Conclusion The inflammatory cytokine in hippocampus of POFS rats is increased and the inflammation signal pathway is activated. Ginsenoside Rbl has some improvement effects on central fatigue in POFS rats.