HPLC法同时测定不同产地紫苏叶和荆芥中咖啡酸和迷迭香酸

Determination of cafferic acid and rosmarinic acid in Perilla frutescens leaves and Schizonepeta tenuifolia by HPLC

目的建立同时测定紫苏Perilla frutescens叶和荆芥Schizonepeta tenuifolia中咖啡酸、迷迭香酸的方法。方法采用反相高效液相色谱法，Agilent C18色谱柱（250mm×4．6mm，5um），流动相为乙腈．0．1％磷酸水溶液，梯度洗脱。检测波长327nm，柱温30℃，体积流量1．0mL／min。结果咖啡酸、迷迭香酸2种成分达到了较好的基线分离，且峰形良好，分别在0．69～22．12、3．65～116．92gg／mL内线性关系良好，r分别为0．9995、1．0000；平均加样回收率分别为紫苏叶102．9％（RSD1．8％）、105．6％（RSD1．9％），荆芥101．2％（RSD2．4％）、101．O％（RSD1．9％）。结论本方法快速、灵敏、准确，所有待测组分峰分离度良好，可为紫苏叶、荆芥药材的质量评价提供依据。

Objective To establish an HPLC method for the simultaneous determination ofcafferic acid and rosmarinic acid in Perillafrutescens leaves and Schizonepeta tenuifolia. Methods The anaiysis was carried out on an Agilent C18 column （250 mm × 4.6 mm, 5 p,m）, and the mobile phase was composed of actonitrile and 0.1% phosphoric acid aqueous with gradient elution. The detection wavelength was 327 nm. The flow rate 1.0 mL/min at column temperature of 30 ℃. Results Under the chromatographic condition, cafferic acid and rosmarinic acid were completely separated and other components had no effect on their determination. It showed a good linearity in the range of 0.69-22.12 and 3.65 -116.92 μg/mL. The average recoveries of P.frutescens leaves were 102.9% and 105.6%, and the RSD values were 1.8% and 1.9%. The average recoveries ofS. tenuifolia were 101.2% and 101.0% and the RSD values were 2.4% and 1.9%. Conclusion The established method is rapid, sensitive, accurate, and the test component peak separation degree is good, and could be used for the simultaneous determination of cafferic acid and rosmarinic acid in P frutescens leaves and S. tenuifolia, which provides a scientific basis for the quality evaluation of P. frutescens leaves and S. tenuifolia.