全反式维A酸修复小鼠光老化皮肤的作用及其机制

The Efficacy and Mechanism of Repairing Photoaged Skin by All-trans Retinoic Acid

目的研究全反式维A酸(at-RA)对小鼠光老化皮肤的修复作用,并联合应用维A酸受体激动剂和拮抗剂,初步探讨at-RA改善胶原过程中其受体的作用。方法建立ICR小鼠光老化模型,按照实验分组进行at-RA及其受体激动剂和拮抗剂的干预,通过小鼠皮肤及标本HE染色观察at-RA对小鼠光老化皮肤的修复,采用免疫组织化学方法检测真皮胶原蛋白的表达。结果 RAR激动剂具有类似at-RA的修复小鼠光老化皮肤及增加Ⅰ型和Ⅲ型胶原蛋白表达的作用(P<0.05),而RXR激动剂无此作用(P>0.05);RAR拮抗剂能够拮抗at-RA和RAR激动剂对光老化皮肤的修复作用及胶原蛋白的改善作用(P<0.05),而RXR拮抗剂无此作用(P>0.05)。结论 at-RA和RAR激动剂均具有显著修复小鼠光老化皮肤并增加Ⅰ型和Ⅲ型胶原蛋白表达的作用,维A酸受体机制参与此过程,其中RAR起重要作用。

Objective With the intervention of all-trans retinoic acid （at-RA）, retinoic acid receptor agonists and an- tagonists, we aimed to investigate whether retinoic acid receptor contributed to improving the photoaging by at-RA through testing the collagen change of the mice skin. Methods The ICR mice photoaging model were topically treated with at-RA, RAR （retinoic acid receptor） or RXR（retinoid X receptor） agonist/antagonists separately. The changes of the mice skin were analyzed through macroscopical observation, HE staining, and the immunohistochemistry detection of collagen（type I and HI ） . Results Both at-RA and RAR agonists significantly repaired the photoaged skin and increased dermal collagen （ P 〈 0. 05 ）, while RXR agonists didn ＇ t （ P 〉 0. 05 ）. RAR antagonists could antagonize the skin repairation and collagen improvement by at- RA and RAR agonists （ P 〈0. 05）. Conclusion These results demonstrate that the effect of both at-RA and RAR agonists in repairing the photoaged skin and increasing the collagen may be related to retinoic re- ceptor mechanism, in which RAR is likely to play a key role.