摘要
目的探讨活性维生素D对2型糖尿病大鼠肾组织胰岛素相关信号分子胰岛素受体(IRβ)、磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(Akt/PKB)表达的影响。方法 30只雄性Wistar大鼠随机分为普通饲料组即正常组(N组,n=10)和高脂高糖饲料组(n=20),高脂高糖饲料组以小剂量(30 mg/kg)链脲佐菌素(STZ)加高糖高脂饮食诱导2型糖尿病模型后随机分为2组,分别经腹腔注射2.5μg/(kg·d)花生油即糖尿病组(M组,n=10)或腹腔注射2.5μg/(kg·d)溶于花生油中的活性维生素D即维生素D治疗组(D组,n=10)。8周后留取大鼠血标本,测定血糖、血肌酐及尿素氮,处死大鼠留取肾组织。Western blot检测肾组织中胰岛素相关信号分子IRβ、PI3K、Akt的表达及其磷酸化的水平。结果糖尿病组大鼠血糖、尿素氮、肌酐高于正常组(P<0.05);而相对于糖尿病组,维生素D干预组血糖、尿素氮及肌酐明显降低(P<0.05)。Western blot显示相较于正常组,糖尿病组大鼠肾组织总的IRβ、Akt的表达无明显改变而其磷酸化水平及PI3K的水平明显下调,而维生素D干预后明显上调(P<0.05)。结论活性维生素D可激活胰岛素信号通路PI3K/Akt的表达,对糖尿病大鼠肾脏损伤发挥保护作用。
Objective To explore the effect of 1,25-dihydroxyvitamin D3 on PI3K/Akt signal pathway in kidney tissues of type 2 dia- betic rats. Methods Thirty Wistar rats were randomly divided into normal group（n = 10） and experimental group（n = 20）, Wistar rats in experimental group were injected with low dose of streptozotocin（25 mg/kg） and fed with diets enriched in both glucose and fat to induce type 2 diabetic model, and then divided into 2 subgroups randomly（ n = 10 in each group）. The diabetic rats were intraperito- neally injected with 1, 25-dihydroxyvitamin D3 （vitamin D group） or peanut oil（ diabetic group）. All rats were killed at 8 weeks to de- tect the levels of blood sugar（BS）, blood urea nitrogen（BUN） and serum creatinine（Scr）. The expression levels of p-IR,p-Akt and PI3K were detected by Western blot. Results Compared with normal group, the levels of BS, BUN and Scr were increased. Those expression levels were lower in vitamin D group than in diabetic group. The result of Western blot showed that the expression of p-IR, p-Akt and PI3K in diabetic group reduced significantly compared with normal group, whereas the expression increased significantly af- ter vitamin D treatment （P 〈 0.05 ）. Conclusion The 1,25-dihydroxyvitamin D3 has a protective effect on the renal function of type 2 diabetic rats via activating the insulin signal pathway PI3K/Akt.
出处
《山西医科大学学报》
CAS
2015年第7期617-620,共4页
Journal of Shanxi Medical University
基金
宜昌市科技攻关基金资助项目(A13301-08)
