摘要
为研究早胜牛(Bos taurus)A-FABP基因多态性与胴体品质和肉质性状的相关性,采用PCR-SSCP方法对5个早胜牛群体(庆阳群体、平凉群体、南德温与庆阳杂种群体、西门塔尔与平凉杂种群体、秦川与平凉杂种群体)A-FABP基因型进行了测定,并分析其多态性与胴体品质和肉质性状的相关性。结果表明,A-FABP基因第2外显子区c.280A>G,并检测到3种基因型AA、AG和GG。对屠宰后胴体和肉质性状相关分析表明,基因型AG屠宰率极显著高于AA(P<0.01),而净肉率GG极显著高于AA(P<0.01);基因型AG剪切力显著低于AA(P<0.05)。由此认为A-FABP基因突变位点可作为早胜牛胴体性状和肉质性状遗传标记。
In order to study the polymorphism of A-FABP gene and its correlation with the characteristics of carcass and meat quality in Zaosheng cattle（Bos taurus）,the SNPs of A-FABP gene in five groups of Zaosheng cattle（Qingyang,Pingliang,Hybrid of Qingyang with South Devon,Hybrid of Simmental with Pingliang and Hybrid of Qinchuan with Pingliang）were detected using PCR-SSCP and their characteristics of carcass and meat quality were analyzed.The results showed that there was a base mutation at c.280A〉G of A-FABP gene in exon 2which lead to three genotypes AA,AG and GG.The statistical correlation analysis indicated that dressing percentage of AG genotype was extremely significantly higher（P〈0.01）than that of AA genotype.Percentage of net meat in GG genotype was extremely significantly higher（P〈0.01）than that of AA genotype.The WBSF of AA genotype was significantly higher（P〈0.05）than that of AG genotype.These results suggested that the site mutation of A-FABP gene could be considered as maker to carcass and meat quality.
出处
《草业科学》
CAS
CSCD
北大核心
2015年第7期1150-1155,共6页
Pratacultural Science
基金
甘肃省高等学校科研项目(2013A-069)
