固定配方加引经药牛膝对防治兔股骨头坏死的作用机制研究

Effects and Mechanism Research on the Fixed Recipe Plus Channel Ushering Drug of Achyranthes Bidentata on the Prevention and Treatment of Rabbit Femoral Osteonecrosis

目的:探讨中药固定配方补气生骨方加引经药牛膝对兔股骨头坏死的修复作用并对其机制进行初步研究。方法:通过大肠杆菌内毒素联合氢化泼尼松龙制作激素性兔股骨头坏死模型,用补气生骨方并分别加引经药牛膝和独活治疗股骨头坏死,观察血管分布、组织病理学变化,检测血清中IGF-1含量及血管VEGF的表达。结果:2周、4周、8周时牛膝组血管面积与模型组比较增加,且差异有统计学意义,均大于固定配方组与独活组。病理学观察该方加引经药牛膝可使骨小梁间隙降低,厚度和体积增加,空骨陷窝率下降,与模型组比较差异有统计学意义,均小于固定配方组和独活组,并可明显提高血清中IGF-1含量以及VEGF的表达水平。结论:补气生骨方加引经药牛膝可通过提高血清中IGF-1含量以及VEGF的表达水平来促进股骨头坏死的修复,其中引经药对于药物作用的发挥起到了重要引导作用。

Objective： To study the effect and mechanisms of fixed formula with channel ushering drug-achyranthes on the osteonecrosis of the femoral head. Methods： The hormone-induced osteonecrosis of femoral head model was made by Escherichia coli endotoxin and hydrogenated prednisolone in rabbits,and they were treated with replenishing Qi and producing bone formula combining with different channel ushering drugs-achyranthes and pubescent angelica. The vascular distribution and histopathological changes were observed,the level of IGF-1 in serum and the expression of VEGF were detected. Results： The vascular area of achyranthes group was increased and was significantly different when compared with model group at the 2nd,4thand 8thweeks. It was also higher than the fixed formula group and pubescent angelica group.Histopathological observations showed that the mean space of trabecular bone reduced and thickness and volume increased.The ratio of empty lacuna declined significantly when compared with model group,and was smaller than fixed formula group and pubescent angelica group. The level of IGF-1 in serum and the expression of VEGF were increased significantly.Conclusion： The fixed formula with achyranthes can promote the healing of osteonecrosis of the femoral head by increasing the levels of IGF-1 in serum and the expression of VEGF,in which the channel ushering drug achyranthes plays an important guiding role.