HPLC-UV法测定薏苡仁指标性成分甘油三油酸酯含量

Quantitation of triacylglycerols in coix seed using HPLC-UV

目的：建立高效液相色谱-紫外法测定薏苡仁指标性成分甘油三油酸酯含量的方法。方法：采用Microsorb C18色谱柱（4.6mm×250mm,5μm）,以乙腈-异丙醇（60∶40）为流动相,流速为1m L·min-1,检测波长为205nm,柱温为20℃。结果：薏苡仁指标性成分甘油三油酸酯的线性范围为5.998-59.98μg（r=0.9993）,平均回收率（n=6）为100.50%（RSD=1.307%）。结论：本法采用高效液相色谱,结合常规紫外检测器,建立了一种准确可靠,通用性较强的薏苡仁中甘油三酯的含量测定方法。

Objective： To establish a quantitation of triacylglycerols in Coix seed by high performance liquid chromatography coupled with UV detector. Methods：Microsorb C18 column（4.6mm×250mm, 5μm）, acetonitrile and iso-propanol （60∶40）as mobile phase, the flow rate is 1mL·min^-1, the detection wavelength was 205nm, the col-umn temperature was 20℃. Results：The linear range of triacylglycerols was 5.998-59.98g（r=0.9993）, the average recovery rate was （n=6）100.50% （RSD=1.307%）. Conclusion：A simple, accurate and reproducible HPLC-UV method was established for determination triacylglycerols in Coix seed.