超声辅助提取藤梨根总黄酮工艺研究

Ultrasound-assisted Extraction of Radix Actinidiae Total Flavonoids

本实验通过超声辅助乙醇的方法提取藤梨根中的总黄酮,先用分光光度计测定提取液中总黄酮的吸光度,然后计算出其提取率,以总黄酮提取率为指标,采用单因素实验确定实验各因素水平,设计正交实验,最后将正交实验结果进行极差分析和方差分析。得出结果:各因素对提取率的影响顺序为:料液比﹥乙醇浓度﹥超声功率﹥温度,在乙醇浓度为60%、温度为60℃、功率为140W、料液比为1:25的条件下提取率最高为9%。

In this experiment, we extract total flavonoid in Radix Actinidiae by ethanol with ultrasound wave. Measure total flavonolds＇s absorbancy by spectrophotometer, Then, calculate it＇s extract efficiency, Taking the total flavonoids ＇s extract efficiency as evaluation criteria, single factor test are used to determine the each factor level. Then, Design a orthogonal test, Finally, Results analysis of variance and range to arrive at such conclusion ： The order of extract efficiency influence with factors is： ratio of material 〉 ethanol concentration 〉 ultrasonic power 〉 temperature. And under the condition of ethanol concentration 60% ,temperature 60℃, power 140W and the ratio of material to solvent 1：25, the extract efficiency is supreme and extraction rate will be 9 %.