高压氧对人脐带华通胶间充质干细胞移植治疗大鼠心肌梗死的效应性研究

Study on the therapeutic effects of hyperbaric oxygen on human umbilical cord Wharton jelly mesenchyme cells on myocardial infarction rats

目的 探讨高压氧是否能有效提高干细胞治疗急性心肌梗死的移植效应.方法 70只SD大鼠按随机数字表法分为假手术组、心肌梗死（AMI）组、干细胞移植（MSCs）组、高压氧（HBO）组、干细胞移植＋高压氧（MSCs＋ HBO）组.建立大鼠心肌梗死模型,模型建立后第1天进行高压氧治疗30 d.心肌梗死后第3天进行人脐带华通胶间充质干细胞移植,于干细胞移植后第7天行心肌组织HE染色观察梗死心肌组织病理性变化,干细胞移植后第4周行EDU染色观察移植干细胞的存活及增殖情况,经胸超声心动图检查观察各组心功能状况.结果 （1）干细胞移植后第4周,MSCs＋HBO组与MSCs组比较,心肌组织局部干细胞存活增殖率显著增高（276.6±73.8 vs 20.5 ±12.3,P＜0.01）;（2）干细胞移植后第7天,AMI组及MSCs组心肌梗死面积及炎性细胞浸润数量均显著高于HBO组及MSCs＋ HBO组（P＜0.01）;（3）心肌梗死后4周,HBO、MSCs、MSCs＋ HBO组较AMI组心功能各指标均有不同程度改善,其中改善最为显著者为MSCs＋ HBO组（P＜0.01）,但除外舒张末期容积（P＞0.05）.结论 高压氧辅助干细胞移植治疗大鼠心肌梗死,心肌炎性损伤显著减轻,心功能明显改善,则提示高压氧能够增强干细胞在受损心肌局部的增殖活性,从而有效提高干细胞治疗AMI的移植效应.

Objective To explore whether hyperbaric oxygen could enhance the effects of the transplanted stem cell on treating myocardial infarction rats.Methods A total of 70 Sprague-Dawley （SD） Rats was randomly divided into four groups,sham operated,acute myocardial infarction （AMI）,mesenchymal stem cells （MSCs） （Stem Cell Transplanting）,and hyperbaric oxygen （HBO） ＋ MSCs group,respectively.Myocardial infarction rat model was established,hyperbaric oxygen treatment was carried out every day at the first to 30 day after AMI.Human umbilical cord Wharton jelly mesenchyme cell was transplanted at the third day after AMI.The densities of polymorphonuclear neutrophils and macrophages in the infracted border zones and in the infarcted area of heart were determined with hematoxylin and eosin （HE） staining.The cardiac function was assessed with M-mode transthoracic echocardiography,in vivo.Results （1）Compared to MSCs group,the stem cell proliferation and survival rates were increased significantly at the forth week （276.6 ±73.8 versus 20.5 ± 12.3,P 〈0.01）.（2）Compared to the AMI and MSCs groups,the infracted area and the number of inflammatory cell infiltration were significantly reduced in HBO alone and MSCs ＋ HBO groups.（3） Compared to the AMI group,the cardiac function was significantly improved at the different degree in the other three groups;among these groups,cardiac function was significantly improved in the MSCs ＋ HBO group （P 〈0.01）,except for the volume of end-dilation （P 〉0.05）.Conclusions Hyperbaric oxygenation could significantly promote the proliferation and survival rate of stem cells,and improve the cardiac function.Thus further enhance the effect of the transplanted stem cell on treating the infarcted heart.