动物细胞无血清培养基的发展和应用

Progress on serum-free media of zooblast

随着生物医药产业和生命科学基础研究对动物细胞培养规模、效率和安全性能要求的不断扩大和提高,无血清培养基的研制和开发已经成为细胞工程领域的重要课题。无血清培养基的发展历经了无血清来源、无动物源、无蛋白和化学成分限定培养基等四个阶段,逐步使得无血清培养基的成分更为明确和简单,进一步提升了无血清培养基在细胞培养中可避免外源物污染等的优势,使其在生物制品和生命科学领域获得了广泛的应用和发展。本文主要系统总结了无血清培养基的发展历程、组分构成和优化方法,尤其对无血清培养基近年来在生物制品、干细胞培养及肿瘤研究等方面的最新开发应用进行了扼要分析述论,以期对无血清培养基在生物学前沿领域的应用和优化提供可借鉴的方向,尤其在肿瘤干细胞的方面可以进行更深入的探索。

With the expanding scale, efficien culture in biomedical industry and research cy and safety requirements during mammalian cell of life sciences, development of serum-free media （SFM） has become a major task in the field of cell engineering. The technology of SFM has experienced four stages including no serum source, no animal source, no proteins and chemically defined medium, and now reached to a status where SFM has more clear components and is easy- handling, allowing users to avoid the potential contaminants of pathogens from environment during cell culture, therefore, to bring a splendid progress and wide applications in the fundamental research of life sciences and bio-pharmaceutical industry. In this review, the classification, components and optimization of SFM were summarized, and latest applications in biological products, stem cells and cancer researches were briefly discussed. This will be helpful for the exploration of SFM in the research of biology, especially in the aspect of tumor stem cells.