中缝大核微量注射5-HT、IL-6及L-Arg对胃运动的调节作用

Effect of microinjecting 5-HT,IL-6 and L-Arg into nucleus raphe magnus on gastric motility in rat

为了研究中缝大核(Nucleus Raphe Magnus,NRM)对胃运动的调节作用及其机制,实验采用大鼠中缝大核给药,张力传感器记录胃收缩幅度及频率。结果显示:中缝大核微量注入五羟色胺(5-hydroxytryptamine,5-HT)能增强大鼠胃收缩幅度,纳洛酮能抑制5-HT的这一作用;中缝大核内白细胞介素-6(Interleukin-6,IL-6)可以增强胃收缩幅度;L-精氨酸(L-Arginine,L-Arg)可以减弱胃收缩幅度,消除5-HT的强胃效应,反转IL-6的强胃效应;利醅酮可以增强L-Arg效应,消除IL-6的强胃效应。研究表明,中缝大核内的5-HT对胃运动的调节与内源性阿片肽有关,并受一氧化氮(NO)的调制。同时,5-HT也通过2A受体调节NO的作用;NRM内IL-6也参与胃运动的调节,其作用也与NO和5-HT2A受体相关。IL-6、NO和5-HT在NRM组成了一个内部调节网络,相互协同调节胃运动。

In order to study the modulation and mechanism of nucleus raphe magnus on gastric motility in rat, experiments were made by means of microinjecting drugs into nucleus raphe magnus（NRM）and recording the frequency and amplitude of gastric motility. The results showed that injection of 5-HT into NRM could increase gastric motility, and naloxone could inhibit the effect of 5-HT. In NRM,IL-6 could increase gastric motility. L-Arg could decrease the motility, and eliminate the effect of 5-HT, and reverse the effect of IL-6~ Ritanserin could enhance the effect of L-Arg, and abolish it of IL-6. In conclusion, the effect of 5-HT on gastric motility is modulated by endogenous opioid peptides and NO. Additionally, 5-HT2A receptor could also modulate NO function; IL-6 also participates the modulation of NRM in gastric motility, and work together with NO and 5-HT2A receptor. In NRM, 5-HT, IL-6 and NO form a complex mechanism, they cooperated with each other to regulate the gastric motility.