阿魏酸钠对溃疡性结肠炎小鼠肠道NF-kB表达和活性的影响

The effects of sodium ferulate on the expression and the activity of NF-k B of the intestine of ulcerative colitis mice

目的：探讨阿魏酸钠（sodium ferulate, SF）对溃疡性结肠炎治疗的可能相关机制。方法：小鼠随机分4成组空白对照（NC）组、模型（MD）组、低剂量（SFLD）、高剂量（SFHD）阿魏酸钠治疗组，NC组采用正常饮用水饲养，采用5%葡聚糖硫酸钠（Dextran sulfate Sodium, DSS）制作UC小鼠模型，造模7 d成模后分为MD组、SFLD组和SFHD组，予药物干预治疗后续7 d。记录分析疾病活动指数（DAI）及脾脏指数（Spleen Index SI），取结肠组织行HE染色；Western blot检测结肠NF-KB表达水平；ELISA法测小鼠肠粘膜NF-kB活性。结果：SFLD组、SFHD组小鼠第8～14 d DAI明显低于MD组（P＜0.05）；SFLD组、SFHD组小鼠第14 d SI明显低于MD组（P＜0.05）；MD组SI明显高于NC组（P＜0.05）；SFLD组、SFHD组结肠NF-kB表达和活性明显低于MD组；MD组结肠NF-kB表达和活性明显高于NC组（P＜0.05）；MD组NF-kB表达和活性明显高于NC组（P＜0.05）。结论：阿魏酸钠可以通过降低结肠NF-kB表达及活性；抑制脾脏代偿性增大，从而减轻肠道的炎症反应及抑制脾脏的免疫反应，改善UC全身炎症状态及免疫应答，对UC发挥治疗作用。

Objective：To investigate the mechanismof sodiumin the treatmentof ulcerative colitis. [Method] Mice were randomly divided i nto 4 groups：bl ank c ontrol （N C） , model group （MDgroup）, lowdo se SF group（SFLD）, high dos e SF group （SFHD）. N C group with normal drinkingwater feeding, After 7 days of using5%dextran sulfate sodiumto make UC mouse model, the models with 7 days of drugi ntervention were divided into MDgroup, SFLD group and SFHD group. Re cord and a nalyze the di sease a ctivity index （DAI） and spleen index （Spleen Index SI）, colon tissue were stained with HE;NF-KB expression of colon were detected by western blot;NF-kB a ctivity of intestinal mucosal were measured by E LISA. R esult：AmongSFLD group and SFHD group of mice on d ays 8-14, DAI was s ignificantly l ower than that of MDgroup （ P〈0.05）; AmongSFLD group and SFHD group of mice on 14 da ys, SI was significantly lower than thatof MD group（P〈0.05）;the SI of MD group was significantly higher than thatof group NC （P〈0.05）;AmongSFLD group and SFHD group, the expression and activity of NF-kB in colon was significantly lower than thatof MD group;the active of NF-kB expression in MD group was significantly higher than thatin NC group （P〈0.05）;the activity of NF-kB expression in MD group was higher than thatin NC group （P〈0.05）. Conclusion：Sodiumferulate can reduce the expression and activity of NF-kB of colon, inhibitthe s pleen’s c ompensatory increase, r elieve the i nflammatory r eaction of intestine,alleviate i mmune r eaction of s pleen,improve sy stemic inflammatory state and immune response of UC and play a role in the treatmentof UC.