营养钵基部浸泡盐液法鉴定葡萄砧木的耐盐性及其生理响应

Evaluating the Salt Tolerance of Grape Rootstocks by Base Soaking of the Plants with Salt Solution

以12个葡萄砧木品种的营养袋扦插苗为试材,100mmol/L NaCl胁迫21d后,对盐害指数、新梢长度、叶片数、根数、根长、根的干鲜质量、新稍干鲜质量等生长指标以及MDA,Pro,WSS,SOD活性等生理指标进行测定.结果表明:盐胁迫下,不同葡萄砧木新梢和根系干鲜质量较对照均显著降低,新梢鲜质量抗逆系数(ARC)与盐害指数呈极显著负相关(p<0.01),新梢干质量ARC、根系干质量ARC与盐害指数呈显著正相关(p<0.05),根冠比ARC与盐害指数呈显著正相关(p<0.05),叶片中Pro含量与盐害指数呈显著正相关(p<0.01);最终确定101-14耐盐性强,贝达,110R,8B,5A耐盐性中等,山河1号,5BB,3309,山河3号,5C耐盐性弱,SO4和188-08耐盐性极弱.

Twelve grape rootstock varieties cultured in nutrition pots were treated with NaCl at 100 mM by base soaking for 21 days,and growth indicators（salt injury index,shoot length,leaf number,root number,root length,and fresh and dry weight of the roots and shoots）and physiological indicator（MDA,Pro,WSS and SOD）were recorded.Based on adversity resistance coefficient（ARC）and cluster analysis,the salt tolerance of these varieties were comprehensively evaluated.Compared with the untreated control,the dry and fresh weight of the shoots and roots of all varieties significantly declined under salt stress.Salt injury index（SI）was in a highly significant negative correlation with ARC of shoot fresh weight（p〈0.01）,in significant positive correlation with ARC of dry weight of shoots and roots and of root/shoot ration（p〈0.05）,and in a highly significant positive correlation with proline content in the leaves（p〈0.01）.The variety 101-14 showed high tolerance to salt stress,Beta,110 R,8Band 5Ashowed medium tolerance to salt stress,AR1#,5BB,3309,AR3# and 5Cwere shown to be sensitive to salt stress,and SO4 and 188-08 were highly sensitive to salt stress.