毛细管区带电泳法测定替吉奥胶囊含量

Determination of the Contents of Tijiao Capsule by Capillary Zone Electrophoresis

采用毛细管区带电泳法测定替吉奥胶囊中的替加氟、吉美嘧啶和奥替拉西钾的含量。采用弹性石英毛细管柱为分离柱,以pH 11.8的20mmol·L^-1磷酸氢二钠溶液为运行缓冲溶液,分离温度为20℃,分离电压为30kV,测定波长为240nm。替加氟和奥替拉西钾在50-500mg·L^-1,吉美嘧啶在14.5-145mg·L^-1范围内呈线性,检出限分别为1.0,2.3,0.6μg·L^-1。3种化合物的加标回收率在98.2%-100%之间,测定值的相对标准偏差（n=9）在0.4%-0.9%之间。该方法用于替吉奥胶囊的分析,测定值与标示值相符。

A method of capillary zone electrophoresis was developed for the determination of ftoafur,gimeracil and potassium oxonate in Tijiao capsules.An elastic quartz capillary tube was used for separation at 20℃and the separate voltage of 30 kV with a running buffer of Na2HPO4（2 mmol·L^-1,pH=11.8）.The detection wavelength was 220 nm.The linearity ranges of ftoafur and passium oxonateot were 50-500 mg·L^-1 and the linearity range of gimeracil was 14.5-145 mg·L^-1.The detection limits were 1.0,2.3,0.6μg·L-1,respectively.The recovery rates measured by standard addition method were in the range of 98.2%-100%,and RSD（n=9）were in the range of 0.4%-0.9%.The method was used for analyzing Tijiao capsules,giving results in agreement with the indicated value.