自源型微生物在地基渗漏封堵过程中的群落变化分析

ANALYSIS OF THE CHANGES OF MICROBIAL COMMUNITIES IN PROCESS OF BIOSEALING GROUNDS

微生物诱导的渗漏封堵技术是通过对土中微生物进行诱导,使菌落构成发生变化,使产生的代谢产物发生改变,达到改变土的渗透性质,实现对地基渗漏进行封堵的目的。该技术已经被成功应用于一些地下建筑设施防渗工程中,如隧道、储水设施等,对其进行渗漏修补。但该技术仍属于经验性技术,还需要对其机理进行研究,充分了解土、微生物及诱导营养液之间所发生的一系列化学生物学反应,以及它们相互作用对地基土存在的渗透处封堵的基本规律。通过试验对实现有效封堵的砂柱中微生物的群落变化进行分析,包括封堵前后微生物的数量、代谢类型以及微生物种类,为该技术能更好地在实际工程中应用提供参考。

Microbial-induced clogging is a method of leakage repair, with which the composition of strains in a soil can be changed through induction of microbes, thus changing the produced metabolites, which can finally change the permeability of the soil and stop the seepages of grounds. This technology has been successfully applied to the leakage repair of some underground seepage control projects, such as tunnel, storage facilities. But the technology still belongs to an empirical technique, the mechanism of the series of chemical biology reactions and the relationship between microbiology and soil need to be studied. In the study, nutrient solutions were injected into sand columns with leakage and the mechanism of clogging, the number of microorganisms and change in microbial communities in different locations of individual sand columns were analyzed.