高效离子色谱法分离、检测低聚果糖

Separation and Analysis of Fructooligosaccharides by High-Performance Anion Exchange Chromatography

采用Carbo Pac PA20柱和脉冲安培检测器的高效阴离子交换色谱建立一种低聚果糖的分析方法,该法可有效分离蔗果型和果果型低聚糖组分,并由此推测出低聚果糖的来源。结果表明,在优化条件下,蔗果低聚糖中蔗果三糖、新蔗果三塘、蔗果四糖和蔗果五糖的回收率分别为98.27%-99.72%、97.32%-102.56%、99.33%-101.81%和97.38%-102.12%。该法的相对标准偏差在0.55%-2.15%。蔗果三糖、新蔗果三塘、蔗果四糖和蔗果五糖的方法检出限分别为0.13、0.19、0.20μg/g和0.41μg/g。蔗果三糖在0.3-18μg/mL、新蔗果三糖和蔗果四糖在0.5-20μg/mL、蔗果五糖在0.8-23μg/mL的范围内,其质量浓度与峰面积线性关系良好,相关系数在0.999 6-0.999 9之间。本方法简单、易于掌握,测定结果准确,适合低聚果糖产品及蛋白粉和奶粉等功能性食品中低聚果糖成分的检测分析。

A high-performance anion exchange chromatographic（HPAEC） method for the analysis of fructooligosaccharide（FOS） has been developed, using a Carbo Pac PA20 column and a PAD detector. This method clearly separated all the GFn and FFn components, which can indicate the origin of FOS. Under the optimal conditions, the 1-kestose（GF2）, neokestose（neo GF2）, nystose（GF3）, 1-fructosylnystose（GF4） components of sc FOS were analyzed in commercial FOS samples with recoveries of 98.27%–99.72%, 97.32%–102.56%, 99.33%–101.81% and 97.38%–102.12%, respectively. The relative standard deviation of the method was in the range of 0.55%–2.15%. The limits of detection（LOD） for GF2, neo GF2, GF3 and GF4 were 0.13, 0.19, 0.20 and 0.41 μg/g, respectively. In the concentration range of 0.3–18 mg/mL for GF2; 0.5– 20 mg/mL for neo GF2 and GF4; and 0.8–23 mg/mL for GF4, there was a good linear relationship between concentration and chromatographic peak area, with correlation coefficients between 0.999 6 and 0.999 9.The new method has been successfully applied to analyze added FOS in commercial functional foods, such as protein powder and milk powder.