以铕-邻苯二甲酸二丙烯酯为荧光探针测定水中痕量甲萘威

Determination of Trace Carbaryl in Water by EuropiumDiallyl Phthalate Fluorescent Probe

以铕（ Eu3＋）-邻苯二甲酸二丙烯酯为荧光探针,建立了一种快速、灵敏测定水中痕量甲萘威的方法。通过高分辨质谱研究铕与邻苯二甲酸二丙烯酯及甲萘威间的络合作用,比较甲萘威与荧光探针结合前后的荧光光谱变化,考察溶液pH值、干扰物质对配合物荧光强度的影响。实验结果表明,Eu3＋与邻苯二甲酸二丙烯酯形成稳定配位数为2的络合物,甲萘威能与该探针形成多元配位复合物,且甲萘威的结合能显著增强Eu3＋-邻苯二甲酸二丙烯酯探针的荧光发光效率。在pH 9.0条件下,采用245/615 nm作为激发/发射波长,在6.25x10-8-2.50x10-6 mol/L浓度范围内溶液的荧光强度与甲萘威的浓度呈良好线性关系,线性相关系数为0.9968,检出限为9.6x10-9 mol/L。水样经乙腈提取后,采用铕（Eu3＋）-邻苯二甲酸二丙烯酯荧光探针进行测定,方法的回收率在91.8%-94.5%之间,相对标准偏差在6.1%以内。本方法适用于环境水样中甲萘威的快速测定。

A rapid and sensitive method for the determination of trace carbaryl in water by using diallyl phthalate-europium （ Eu3＋） as fluorescent probes was developed. The interaction between Eu3＋ and diallyl phthalate and carbaryl was studied by high resolution mass spectrum, and the fluorescence spectra change of complexes before/after binding with carbaryl was also investigated. The influence factors of fluorescence intensity including solution pH and interferent were studied. The results showed that two diallyl phthalate molecules were complexed with one Eu3＋to form stable complexes. Carbaryl could also interact with the probe to form multiple complexes, which significantly increased the fluorescent efficiency of the probe. At pH 9. 0 of solution and by using 245/615 nm as excitation/emission wavelength, the fluorescence intensity showed good linear relationship with the carbaryl concentration ranged from 6. 25x10-8 mol/L to 2. 50x10-6 mol/L, and the linear correlation coefficient was 0. 9968. The detection limit of the method was 9. 6x10-9 mol/L. Water samples were extracted by acetonitrile, and then detected by europium （ Eu3＋）-diallyl phthalate fluorescent probe. The recovery of the method was 91. 8%-94. 5%, while RSD was within 6. 1%. The method is suitable for the rapid determination of carbaryl in water samples.