天山雪莲乙醇提取物对缺氧小鼠肝脏线粒体的保护作用研究

Protective Effect of Saussurea involucrata Alcohol Extract on Liver Mitochondria in Mice Under Hypoxia Condition

目的:研究天山雪莲乙醇提取物对缺氧小鼠肝脏线粒体的保护作用。方法:将BALB/c小鼠随机分为4组:正常对照组、缺氧模型组、乙酰唑胺阳性对照组、天山雪莲提取物组,给药后将小鼠放入低压氧舱,减压至模拟海拔8 000 m高度,小鼠保持在此高度下缺氧12 h后处死小鼠,提取肝脏线粒体。测定线粒体膜电位、线粒体复合物Ⅰ、Ⅱ、Ⅴ和苹果酸脱氢酶、顺乌头酸酶、α-酮戊二酸脱氢酶、丙酮酸脱氢酶的活性。结果:与缺氧模型组比较,天山雪莲提取物能够保护线粒体膜电位,提高顺乌头酸酶、α-酮戊二酸脱氢酶、丙酮酸脱氢酶和线粒体复合物Ⅰ、Ⅱ、Ⅴ的活性。结论:天山雪莲乙醇提取物能够保护缺氧小鼠肝脏线粒体功能。

Objective： To study the protective effect of Saussurea involucrata alcohol extract on liver mitochondria in mice under hypoxia condition. Methods： The hypoxia mice model was established,the BALB / c mice were randomly divided into four groups： normal group,hypoxia model group,positive control group and Saussurea involucrata alcohol extract group. Mice were put into low pressure oxygen chamber and decompressed,adapted to hypobaric hypoxia environment of simulated altitude of 8 000 m for 12 h,and then recovered to normal altitude. The mice were sacrificed and the liver mitochondria was isolated,the mitochondrial membrane potential and the activity of malate dehydrogenase,aconitase,α-ketoglutarate dehydrogenase,pyruvate dehydrogenase and mitochondrial complex Ⅰ,Ⅱ and Ⅴwere measured. Results： Compared with hypoxia model group,Saussurea involucrata alcohol extract protected mitochondrial membrane potential,sustained the activities of aconitase,α-ketoglutarate dehydrogenase,pyruvate dehydrogenase,and mitochondrial complex Ⅰ,Ⅱand Ⅴ under hypoxia condition. Conclusion： Saussurea involucrata alcohol extract can protect the liver mitochondrial function in mice under hypoxia condtion.