补肾中药复方对老年大鼠尿道功能和膀胱逼尿肌β-AR的影响

Effects of Suoquan Wan and Shenqi Wan on Urethra Function and β-AR Function of Detrusor in Natural Aged Rats

目的:观察中药复方缩泉丸和金匮肾气丸对老年大鼠尿道功能和逼尿肌β-AR的影响。方法:实验分为4组:正常对照组(青龄大鼠)、模型组(老龄大鼠)、缩泉丸组和肾气丸组,每组12只动物。缩泉丸和肾气丸组给予相应浓度的药液,正常对照组和模型组灌胃给予等体积蒸馏水,连续给药6 w。检测缩泉丸和肾气丸对老年大鼠离体逼尿肌应力舒张功能和尿道压的影响,并考察其对β-AR激动剂及β3-AR拮抗剂作用下离体逼尿肌功能的影响。结果:与青年大鼠比较,老年大鼠离体逼尿肌顺应性变大;且对β3-AR激动剂BRL37344和β-AR激动剂异丙肾上腺素(ISO)引起的最大松驰效应(Emax)下降,故药物内在活性(IA)显著降低(P<0.01);其β3-AR拮抗剂的竞争性拮抗强度(PA2)也显著升高(P<0.05)。与模型组比较,缩泉丸和肾气丸均能显著降低膀胱顺应性(P<0.05);显著升高β3-AR激动剂BRL37344及β-AR激动剂ISO作用下逼尿肌条的Emax、IA、PD2(P<0.05或P<0.01);显著降低其对β3-AR拮抗剂的PA2(P<0.05)。老年大鼠最大尿道关闭压(MUCP)、最大尿道压(MUP)和功能性尿道长度(FUL)显著低于青年大鼠(P<0.05);缩泉丸及肾气丸均能增加老年大鼠的MUCP、MUP,肾气丸能增加老年大鼠FUL(P<0.05或P<0.01)。结论:缩泉丸和肾气丸对老年大鼠逼尿肌β-AR功能有显著影响,能改善膀胱逼尿肌的功能。

Objective： To investigate the effect of Suoquan Wan（ SQW） and Jinkui Shenqi Wan（ JKSQW） on urethra function andβ-AR function of detrusor in natural aged rats. Methods： young rats（ 3 months） and Aged rats（ 15 months） were chosen. Young rats were chosen as control,aged rats were randomly divided into three groups： model,JKSQW and SQW groups,12 rats in each group. JKSQW and SQW were given the appropriate concentration once a day for six weeks. The effects of SQW and JKSQW on relaxation function of bladder detrusor was investigated,and their effects on bladder induced by β-AR agonist and β3-AR agonist were further studied. Then,their effects on pressure of urethra were observed. Results： Compared with the young group,detrusor compliance of natural aged model rats was increased,Emaxand IA of agonist of β3-AR including BRL37344 and ISO were decreased（ P〈0. 01）,while PA2 of antagonist ofβ3-AR were increased（ P〈0. 05）. Compared with model group,SQW and JKSQW decreased the bladder compliance（ P〈0. 05）,and increased Emax,IA and PD2 due to sensitivity of detrusor to agonist of β-AR and β3-AR including BRL37344 and ISO（ P〈0. 05 or P〈0. 01）,while decreased PA2 of antagonist of β3-AR（ P〈0. 05）. MUCP,MUP and FUL of aged rats were lower than those of normal rats. But SQW and JKSQW increased MUCP and MUP,and JKSQW increased FUL of aged rats（ P〈0. 05 or P〈0. 01）. Conclusion：SQW and JKSQW can remarkably adjust β-AR function on the detrusor and improve the closure ability of bladder detrusor of the natural aged rats.