益母草总碱对老龄大鼠前列腺增生模型的影响

Effect of Leonuri Herba alkaloids on prostatic hyperplasia in older rats

目的探讨益母草总碱对老龄大鼠前列腺增生模型的作用及相关机制。方法采用雌雄激素诱导法复制老龄大鼠前列腺增生模型,将造模大鼠随机分为5组,分别ig 50.0、25.0、12.5 mg/kg益母草总碱溶液,阳性对照组给予30 mg/kg癃闭舒混悬液,模型组给予同体积的生理盐水,另设老龄大鼠及青年大鼠各1组为对照,ig同体积生理盐水,每天给药1次,连续给药30 d;实验结束测定大鼠的前列腺湿质量,计算前列腺指数;检测血清中雌二醇(E2)和前列腺组织中双氢睾酮(DHT)、睾酮(T)及前列腺组织中碱性成纤维细胞生长因子(b FGF)、转化生长因子-β1(TGF-β1)、表皮细胞生长因子(EGF)、胰岛素样生长因子-1(IGF-1)表达,光镜及电镜观察大鼠前列腺组织形态及超微结构的变化。结果前列腺增生模型复制成功,与模型组比较,益母草总碱低剂量组可明显降低大鼠前列腺湿质量及前列腺指数(P<0.05),益母草总碱中剂量组可明显降低大鼠前列腺指数(P<0.05);益母草总碱各剂量组可显著降低模型大鼠前列腺中的T及DHT水平(P<0.01),以及前列腺组织中b FGF、EGF、IGF-1表达(P<0.05、0.01),益母草总碱低剂量组可显著升高前列腺组织中TGF-β1的表达(P<0.01);益母草总碱各剂量组可显著降低模型大鼠前列腺的体密度(P<0.05、0.01),显著增加前列腺比膜面及比表面值(P<0.01),可使模型所致的前列腺细胞胞浆内线粒体显著减少,减轻线粒体嵴等的病理变化。结论益母草总碱对雌雄激素诱导法所致的老龄大鼠前列腺增生模型有较好的治疗作用。

Objective To investigate the pharmacological effects and mechanism of Leonuri Herba alkaloids （LHA） on prostate hyperplasia in older rats. Methods The prostate hyperplasia model in older rats was induced by male and female hormone, the rats were divided into five groups： 50.0, 25.0, 12.5 mg/kg alkaloids dosage group, 30 mg/kg Longbishu Capsule suspension group, and model group given the same volume of saline. Otherwise aged rats and young rats were set as control groups administered with saline, and administered once daily for consecutive 30 d. The wet weight and prostate index in rat prostate were measured, and the expression of E2, DHT, T, bFGF, TGF-β1, EGF, and IGF-1 in serum and prostate tissue were detected after the experiment. The rat prostate tissue morphology changes and rat prostate cells ultrastructural changes were observed by light microscopy and transmission electron microscopy. Results Compared with the model group, benign prostatic hyperplasia rat model was replicated successfully, low-dose LHA can significantly reduce the rat prostate wet weight and prostate index （P 〈 0.05）, mid,dose LHA can significantly reduce the rat prostate index （P 〈 0.05）. Each dose of LHA can significantly reduce the levels of T and DHT in prostate model rats （P 〈 0.01）. Each dose of LHA can significantly reduce the expression of bFGF, EGF, and IGF-1 in prostate model rats （P 〈 0.01）. Low-dose LHA can significantly increase the expression of TGF-β1 in prostate tissue. Each dose of LHA can significantly reduce the density of prostate model rats （P 〈 0.05, 0.01）, and significantly increase the membrane surface and surface area values （P 〈 0.01）. Each dose of LHA can significantly reduce intracytoplasmic mitochondria in prostate cells caused by model, and relieve the pathological changes of mitochondria cristae. Conclusion LHA has good therapeutic effect to prostatic hyperplasia model rats induced by male and female hormone.