象皮HPLC指纹图谱研究

HPLC fingerprint of Chinese materia medica elephant skin

目的建立象皮HPLC指纹图谱分析方法,为其质量控制以及开发应用提供实验依据。方法用6 mol/L盐酸溶液水解象皮中的氨基酸,以异硫氰酸苯酯(PITC)为柱前衍生剂,用Hypersil ODS-2 C18色谱柱(250 mm×4.6 mm,5μm),梯度洗脱,体积流量为1.0 m L/min,检测波长为254 nm,柱温为40℃。利用"中药色谱指纹图谱相似度评价系统2004A版"对其进行相似度评价。结果建立了象皮HPLC指纹图谱共用模式,确定18个共有峰,并根据对照品指认17个共有峰,10批象皮药材具有较高的相似度,可反映象皮所含的17种氨基酸成分。结论首次建立了象皮的指纹图谱分析方法。该分析方法简便、快速,可为象皮质量的全面控制提供科学依据。

Objective To establish an HPLC method for the fingerprint analysis on Chinese materia medica （CMM） elephant skin so as to provide the evidence for the quality control and application of elephant skin. Methods To extract amino acids by the method of hydrolysis in 6 mol/L HCL and use phenyl isothiocyanate （PITC） as the derivating agent. The Hypersil ODS-2 Ci8 column （250 mm × 4.6 mm, 5 μm） was used. Under the condition of gradient elution, the flow rate was 1.0 mL/min, the UV detection wavelength was 254 nm, and the column temperature was 40 ℃. The similarity was analyzed with ＂Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004,A＂. Results The HPLC characteristic fingerprint of elephant skin has been established. A total of 18 common peaks were characterized, and 17 of them were identified by comparing their retention time with reference substances. The result showed that the amino acid constituents had a high similarity. Seventeen kinds of amino acids in elephant skin were detected. Conclusion It is the first time to establish the HPLC fingerprint of elephant skin. The method is simple and quick, which provides the scientific basis for the comprehensive oualitv control of elephant skin.