银纳米微粒对锌卟啉的荧光增强效用研究

Research on Fluorescence Enhancement Effect of Silver Nanoparticles on Zinc Porphyrin

采用波长为407 nm的激光照射以银纳米颗粒作为荧光增强剂的成人全血溶液,研究其对血液中锌卟啉(ZPP)的荧光增强作用。实验结果表明,胶体状态的银纳米颗粒可以显著增强锌卟啉的荧光强度,增强效果最大可以达到5.2倍,且荧光增强效率随银胶加入量的增加呈现先增后减的趋势。分析认为,当溶液中锌卟啉分子和银纳米颗粒之间的距离满足荧光增强条件时,引起荧光强度的增加。加入大量的银胶颗粒时,分子之间作用距离逐渐减小,增强效果发生变化。研究结果表明使用银纳米颗粒可以实现低浓度血样中锌卟啉荧光的增强效应,为提高锌卟啉检测的灵敏度和精度提供有价值的参考。

Adult blood with silver nanoparticles is radiated by laser with wavelength of 407 nm, and the effect of colloidal silver nanoparticles on the fluorescence performance of zinc porphyrin（ZPP） in blood has been investigated. It has been found that colloidal silver nanoparticles can enhance fluorescence intensity of ZPP in low concentration human blood significantly, the highest enhancement ratio is about 5.2. Moreover, with the increase in silver colloid amount, the fluorescence enhancement efficiency increases firstly and then decreases. Theoretical analysis indicates that when the distance between ZPP molecules and silver nanoparticles in the solution meets fluorescence enhancement condition, the intensity of ZPP fluorescence can be improved. However, after adding extra silver particles, the distance between molecules will decrease, which is detrimental to the enhancement effect. The research shows that silver nanoparticles can enhance the fluorescence intensity of ZPP in low concentration human blood, which is important for the improvement of ZPP detection sensitivity and accuracy.