基于高通量测序的文冠果转录组分析

Transcriptome Analysis for Xanthoceras sorbifolia Bunge Based on High-throughput Sequencing Technology

应用Illumina Hi-seqTM2000高通量测序技术对文冠果花芽进行转录组分析。共获得N50为1 180bp、平均长度为686bp的unigene 58 311条。与公共数据库Nr和Swiss-Prot同源性比较后发现37 047条unigene获得基因注释,另有21 264条unigene未被注释。利用COG数据库将unigene分成25类。通过GO分类和KEGG Pathway富集性分析,将unigene分别归类于55个GO term和128个代谢途径。此外,在9 794条unigene中共搜索到12 213个SSR位点,单核苷酸重复基元出现频率最高(34.95%),其次分别为二核苷酸(32.74%)和三核苷酸(28.64%)。在获得的unigene中发掘出涉及4个开花调控途径(光周期途径、春化途径、GA途径和自主途径)多个基因的同源序列。研究结果可在一定程度上解析文冠果花芽形态分化的分子调控模式与机制。

Illumina Solexa Hi-seq 2000 high-throughput sequencing technology was used to get the comprehensive transcriptome from flower buds of Xanthoceras sorbifolia. A total of 58 311 unigenes were generated, with average length and NS0 of 686bp and 1 180bp respectively. Using Blastx against the public databases of Nr and Swiss-Prot, 37 047 unigenes were annotated. 21 264 unigenes were not found in any databases. Unigenes were classified into 25 classes through COG databases. Through GO classification and KEGG pathway enrichment analysis, all of these differentially expressed unigenes were classified into 55 GO terms and 128 metabolism pathways, respectively. In addition, 12 213 potential SSR loci were detected from 9 794 unigene. Of them, occurrence frequency of mono-nucleotide repeats was highest （34.95 % ）, dinucleotide repeat and trinucleotide repeats accounted for 32.74% and 28.64%, respectively. Some unigenes identified from transcriptome data were referred to four major plant flowering regulation pathways including photoperiod pathway, verbalization pathway, GA-dependent pathway and autonomous pathway, to some extent the molecular mechanisms regulating mode with flower bud morphological differentiation of Xanthoceras sorbifolia has been resolved.