Chlorococcum sp.混养过程碳氮调控对累积类胡萝卜素和脂肪酸成分影响研究

Effect of Carbon and Nitrogen Combination on the Cartenoid and Fatty Acids Accumulation of Chlorococcum sp. under Mixotrophic Cultivation

在BG-11培养液的基础上,选择不同的乙酸钠和硝酸钠浓度组合培养绿球藻Chlorococcum sp.,分析培养过程中干重、类胡萝卜素、总脂及脂肪酸成分,完成Chlorococcum sp.生长及油脂积累的评价。实验结果表明,外源碳源乙酸钠对Chlorococcum sp.的生长和油脂积累有很好的促进作用。乙酸钠和硝酸钠浓度分别为2g/L和1g/L条件下,Chlorococcum sp.培养16天后生物质产率高达276.19mg/(L·d),同时类胡萝卜素和总脂的产率也分别高达1.347mg/(L·d)和108.59mg/(L·d),高附加值脂肪酸二十碳五烯酸(EPA)与二十二碳六烯酸(DHA)占脂肪酸总量也超过了1.5%。

The object was to analyze the effects of different combinations of additional carbon and nitrogen resources on the growth and lipid accumulation of Chlorococcum sp. under modified BG-11 culture pretreated by different concentrations of sodium acetate and sodium nitrate. The biomass of Chlorococcum sp. was determined by dry weight method. The features of lipid accumulation were analyzed by lipid productivity, carotenoid productivity and fatty acid methyl ester （FAME） composition. The results showed that Chlorococcum sp. cultivated in the modified BG11 with mass concentrations of sodium acetate 2g/L and sodium nitrate l g/L had significantly improved algal growth and lipid accumulation. The final biomass, carotenoid and lipid productivity reached 276. 19mg/（L · d）, 1. 347mg/（L · d） and 108. 59mg/（L· d）, respectively after 16 days＇ cultivation. It is worth to note that the yield of docosahexaenoic acid （DHA） and eicosapentaenoic acid （EPA） reached 1.58% of total fatty acids.