康妇炎胶囊对兔盆腔炎性疾病后遗症炎症因子影响的实验研究

Experimental research on the effect of Kangfuyan capsule on inflammatory factor of rabbits with sequelae of pelvic inflammatory disease

目的通过观察康妇炎胶囊对兔盆腔炎性疾病（PID）后遗症模型血清白介素（IL）-4、IL-10、IL-8、转化生长因子（TGF）-β1水平的影响,探讨康妇炎胶囊治疗盆腔炎性疾病后遗症的机制。方法将5~6个月龄新西兰雌兔分为对照组、模型对照组和康妇炎组。模型对照组和康妇炎组在逆行宫腔插管灌注细菌法建立盆腔炎性疾病模型成功后,分别灌服蒸馏水和康妇炎胶囊3周。肉眼和HE染色观察各组兔子宫及输卵管情况;酶联免疫吸附法（ELISA）检测各组兔血清IL-4、IL-10、IL-8和TGF-β1水平的变化。结果模型对照组兔腹膜明显增厚,盆腔积液,子宫充血、质脆、弹性差,输卵管管壁粗糙、增厚,与周围组织粘连,或外观略呈串珠样;康妇炎组兔腹膜增厚不明显,无盆腔积液、子宫无明显充血、输卵管与周围组织无明显粘连;康妇炎组兔血清IL-4、IL-10水平较模型对照组明显升高（P〈0.01）,而IL-8、TGF-β1水平较模型对照组明显降低（P〈0.05）。结论康妇炎胶囊可能通过下调前炎症细胞因子、腹腔粘连因子和上调抗炎症细胞因子的表达,调整致炎和抗炎的动态平衡,促使盆腔炎性疾病的愈合。

Objective To investigate the mechanism of Kangfuyan capsule on treating sequelae of pelvic inflammatory disease（PID）of by observing the effect of Kangfuyan capsule on the content of serumal inflammatory cytokines（IL）-4,IL-10,IL-8and TGF-β1of in PID modle rabbits with sequelae of PID.Method New Zealand female rabbits with 5~6month age were divided into normal group,model group,Kangfuyan Capsule group.Except the normal group,rabbits in other 2groups were all established the sequelae of pelvic inflammatory disease（PID）model by injecting mixture of bacterial suspension into rabbits＇ uterus.Model group,Kangfuyan Capsule group were given distilled water,Kangfuyan Capsule for 3weeks respectively after the sequelae of PID models established successfully.The normal group also were given distilled water for 3 weeks.Change of pelvic,uterus and fallopian tubes was observed by naked eyes and HE staining was done to observe the pathological changes.Levels of IL-4,IL-10,IL-8,and TGF-β1were determined by ELISA method.Results In the model group,peritoneal was thickened clearly and pelvic fluid was find frequently was formed.Uterus were bloodshot,brittle and low elasticity.Fallopian tube walls were rough,thickened,had some and adhesion with to the adjacent tissue,and showed with a string-of-beads pattern.In Kangfuyan capsule group,peritoneal was not markedly thickened,no pelvic fluid was found,uterus were no congestion,Fallopian tubes were no adherence with surround-tissue.Compared with model group,serum level of IL-4,and IL-10 content of Kangfuyan capsule group increased significantly（P〈0.01）,while level of IL-8and TGF-β1content decreased significantly（P〈0.05 or 0.01）.Conclusions Kangfuyan capsule could reduce the expression of proinflammatory factors and abdominal adhesions factor and increase the expression of anti-inflammatory cytokines,adjust proinflammatory/anti-inflammatory homeostasis,thus prompt healing pelvic inflammation gradually.