儿童B细胞性非霍奇金淋巴瘤中CHFR的表达及其作用机制

Expression of CHFR in children with B cell non- Hodgkin lymphoma and the mechanism

目的探究儿童B细胞性非霍奇金淋巴瘤(B-NHL)中CHFR(check point with FHA and ring finger)基因表达及其作用机制及临床病理特征。方法选择B-NHL组织90例(B-NHL组)和淋巴结反应性增生组织50例(对照组)作为研究对象,选择荧光定量PCR方法对样本进行检测,对其CHFR基因mRNA表达水平进行评估。结果 1B-NHL组CHFR基因mRNA表达量明显低于对照组,数据差异有统计学意义(t=-3.346,P<0.05);2B-NHL组CHFR基因mRNA表达量在性别、有无全身症状、乳酸脱氢酶水平以及ECOG评分项目中进行比较,各组间数据差异均无统计学意义(P>0.05);3将B-NHL患儿进行Ann/Arbor分期,Ⅰ+Ⅱ期患儿CHFR基因mRNA表达量明显高于Ⅲ+Ⅳ期患儿,差异有统计学意义(P<0.05)。对B-NHL患儿进行IPI评分,0-1分组患儿CHFR基因mRNA表达量明显高于2-5分组,差异有统计学意义(P<0.05)。结论在儿童B-NHL发病过程中,CHFR表达水平发挥一定作用,CHFR作用机制与B-NHL恶性程度及进展具有相关性。

Objective To explore gene expression of check point with FHA and ring finger（ CHFR） in children with B cell non-Hodgkin lymphoma（ B- NHL） and the mechanism,clinicopathological features. Methods Ninety cases of B- NHL（ B- NHL group）and fifty cases of reactive hyperplasia of lymph node（ control group） were included in the study,fluorescent quantitative PCR was used to detect the samples,the expression level of CHFR mRNA was evaluated. Results The expression level of CHFR mRNA in B- NHL group was statistically significantly lower than that in control group（ t =- 3. 346,P〈0. 05）; in B- NHL group,there was no statistically significant difference in the expression level of CHFR mRNA between boys and girls,with systemic symptoms or not,with different lactate dehydrogenase levels,and ECOG scores（ P〉0. 05）; the children in B- NHL group were divided into different stages according to Ann / Arbor staging,the expression level of CHFR mRNA in the children of stage Ⅰ ＋ Ⅱ was statistically significantly higher than that in the children of stage Ⅲ ＋ Ⅳ（ P〈0. 05）. The expression level of CHFR mRNA in children with IPI score 0- 1 was statistically significantly higher than that in children with IPI score 2- 5（ P〈0. 05）. Conclusion CHFR gene expression level plays a certain role in the course of B- NHL in children,there is a correlation between CHFR mechanism and malignant degree and progression of B- NHL.