苯妥英钠对内毒素作用下人牙周膜成纤维细胞增殖和表达TNF-α的影响

Effects of phenytoin on the proliferation and tumor necrosis factor-αexpression in LPS-treated human periodontal ligament fibroblasts

目的:观察苯妥英钠(PHT)对内毒素(LPS)作用下人牙周膜成纤维细胞(h PDLFs)增殖和表达肿瘤坏死因子-α(TNF-α)的影响。方法:原代培养h PDLFs,分别与100 mg/L LPS、不同浓度PHT(5、20 mg/L)以及100 mg/L LPS+不同浓度PHT共同培养24 h后,采用甲基噻唑基四唑(MTT)法观察不同浓度PHT对LPS作用下h PDLFs增殖活性的影响;采用免疫细胞化学SP法检测PHT对LPS作用下h PDLFs内TNF-α的表达情况。结果:100 mg/L的LPS可明显抑制h PDLFs的增殖活性、刺激h PDLFs表达TNF-α(P<0.05);20 mg/L PHT可明显促进h PDLFs的增殖(P<0.05),并可明显抑制h PDLFs表达TNF-α(P<0.05);将20 mg/L PHT与100 mg/L LPS联合作用于h PDLFs时,可明显逆转LPS对h PDLFs增殖(P<0.05),明显拮抗LPS介导h PDLFs表达TNF-α(P<0.05)。结论:20 mg/L PHT对LPS作用下的h PDLFs具有保护作用。

AIM： To study the effects of phenytoin（PHT） on the proliferation and tumor necrosis factor-α（TNF- α） expression in lipopolysaccharide（ LPS）- treated human periodontal ligament fibroblasts（ h PDLFs）.METHODS： h PDLFs were cultured in vitro and identified by immunocytochemistry（ ICC） SP method. The cells were cultured with 100 mg / L LPS,various concentrations of PHT and 100 mg / L LPS plus various concentrations of PHT respectively for 24 h. The cell proliferation was examined by MTT assay. TNF-α expression in the cytoplasm was detected by SP method. RESULTS： 100 mg / L LPS inhibited the proliferation of h PDLFs and increased TNF-α expression in the cytoplasm of h PDFLs. Phenytoin dose- dependently attenuated the effects of LPS on h PDLFs prolifereation and TNF-α expression. CONCLUSION： PHT may have protective effects on LPS- treated h PDLFs.