消乳增胶囊对大鼠胃酸和胃蛋白酶的影响研究

Study on the Effects of Xiaoruzeng Capsule on the Gastric Acid and Pepsase in Rats

目的:研究消乳增胶囊对大鼠胃酸和胃蛋白酶的影响。方法:取大鼠随机分为空白(蒸馏水)组、阳性对照(奥美拉唑3.6mg/kg)组和消乳增胶囊低、中、高剂量[2.25、4.5、9.1 g(生药)/kg]组,依次记为A、B、C、D、E组,每组10只,连续ig给予相应药物10d。末次给药3 h后收集全部胃液,用p H精密试纸检测胃液p H值,用酸碱中和滴定法测定游离酸度和总酸度;用试剂盒和酶标仪检测并计算胃蛋白酶含量和胃蛋白酶活性;电镜下观察胃病理变化。结果:与A组比较,B、C、D组大鼠胃液的p H升高;C、D、E组大鼠胃液游离酸度减小;B、C、D组大鼠胃液总酸度降低,E组大鼠胃液总酸度增加;B、D、E组大鼠胃蛋白酶活性降低;C组大鼠胃蛋白酶含量增加。与B组比较,D、E组大鼠胃液p H降低;C组大鼠胃液总酸度降低,E组大鼠胃液总酸度增加;C、D、E组大鼠胃蛋白酶活性增强。以上差异具有统计学意义(P<0.01或P<0.05)。E组大鼠有3例出现胃黏膜糜烂出血,其余各组无明显病理变化。结论:消乳增胶囊在低剂量时对大鼠胃酸有轻度抑制作用,但不会影响胃蛋白酶活性。

OBJECTIVE：To study the effects of Xiaoruzeng capsule on the gastric acid and pepsin in rats.METHODS：Rats were randomized into a blank group（distilled water）,a positive control group（3.6 mg/kg omeprazole）and the groups of low,middle and high doses of Xiaoruzeng capsule [2.25,4.5,9.1 g（crude drug）/kg].These groups were respectively marked as groups A,B,C,D and E,with 10 rats in each group.All the rats were given corresponding drugs,ig,for consecutive 10 d.Their succus gastricus was collected 3 h after the last administration,and determined for p H value with precision p H test strip and for free acidity and total acidity by acid-base neutralization titration method.The content and activity of pepsin were determined and calculated with the test kit and microplate reader.The pathological change of the stomach was observed under the electron microscope.RESULTS：Compared with group A,groups B,C and D had higher p H value of succus gastricus;groups C,D and E had lower free acidity;groups B,C and D had lower total acidity,group E had higher total acidity;groups B,D and E had lower activity of pepsin;and group C had higher content of pepsin.Compared with group B,group D had lower p H value of succus gastricus;group C had lower total acidity;group E had higher acidity;and groups C,D and E had higher activity of pepsin.There was statistical significance（P〈0.01 or P〈0.05）.Gastric mucosal erosive haemorrhage was noted in three rats in group E,and other groups demonstrated no obvious pathological change.CONCLUSIONS：Low dose of Xiaoruzeng capsule can slightly inhibit the gastric acid in rats,but will not effect the activity of pepsin.