正交设计-多元回归法优选红景天中红景天苷超声波提取工艺

Optimization of Ultrasonic Extraction Technology of Salidroside in Rhodiola rosea by Orthogonal Design-Multiple Regression Method

目的:优选红景天中红景天苷的超声波提取工艺。方法:以红景天苷含量为指标,设计L16(45)正交试验考察乙醇质量分数、料液比、提取时间、超声功率4个因素对红景天提取液中红景天苷含量的影响;采用Matlab 6.5软件,对红景天苷的超声波提取的正交试验数据进行数学模拟,通过多元回归法优选红景天苷的超声波提取工艺,并将优选的工艺与传统的加热回流工艺提取效果进行比较。结果:所得红景天苷最优提取工艺为乙醇质量分数77%、料液比1∶34(g∶ml)、提取时间34 min、超声功率237 W(超声频率40 k Hz)。此条件下提取所得红景天苷含量可达0.982 4%,与预测值0.989 4%相近。与加热回流法相比,两种方法提取所得红景天苷含量相近,但超声波法提取时间缩短了62.2%。结论:超声波提取法提取红景天中红景天苷操作简单、用时较短、提取率较高。

OBJECTIVE：To optimize the ultrasonic extraction technology of salidroside in Rhodiola rosea.METHODS：With the content of salidroside as the index,L16（45）orthogonal test was designed to observe the effects of 4 factors including the mass fraction of ethanol,solid-liquid ratio,extraction time and ultrasonic power on the content of salidroside in R.rosea extraction solution.By using Matlab 6.5 software,mathematical simulation of ultrasonic extraction process of salidroside was made with the data of orthogonal test.Multiple regression analysis method was adopted to optimize the ultrasonic extraction technology of salidroside,and then the extraction result of optimal technology and that of traditional heating reflux technology were compared.RESULTS：The optimal extraction technology of salidroside was as follows as ethanol mass fraction of 77%,solid-liquid ratio of 1 ∶ 34（g ∶ml）,extraction time of 34 min,ultrasonic power of 237 W（ultrasonic frequency of 40 k Hz）.Under the above-mentioned conditions,the content of salidroside was up to 0.982 4%,close to the predicted value of 0.989 4%.Compared to heating reflux method,the ultrasonic method has similar content of salidroside but extraction time was shortened by 62.2%.CONCLUSIONS：The ultrasonic extraction method for extracting the salidroside in R.rosea is simple,requires shorter time,and giving rise to higher extraction rate.