2,4,6-三硝基苯磺酸诱导溃疡性结肠炎大鼠模型的建立及评价

Establishment and Evaluation of Ulcerative Colitis Model on Rats Induced by 2,4,6-Trinitrobenzene Sulfonic Acid

目的建立大鼠2,4,6-三硝基苯磺酸(TBNS)溃疡性结肠炎(ulcerative colitis,UC)模型。方法:大鼠经结肠注入不同剂量的2,4,6-三硝基苯磺酸,分别于给药后1、3及7 d处死大鼠取出结肠,肉眼观察后进行组织学切片然后镜下观察。结果:各组大鼠体质量于造模3 d后开始下降,50 mg·kg-1组体质量于7 d后开始出现回升,100 mg·kg-1组和150 mg·kg-1组体质量则没有恢复;造模时间不是影响UC(溃疡性结肠炎)损伤程度的主要因素;病理切片结果表明,50 mg·kg-1组可见炎性细胞浸润,未出现肠腺肿胀及黏膜脱落现象,符合UC(溃疡性结肠炎)的病理特征。结论:TNBS造模7 d内,UC(溃疡性结肠炎)的造模成功与否与给药天数无关,而与剂量密切关联,以50 mg·kg-1的大鼠给药剂量最为合适。

Objective ：To establish the ulcerative colitis model on rats by 2,4,6 -trinitrobenzene sulfonic acid （TN- BS）. Method：The different doses of TNBS were injected into the colon of rats which were executed after 1 d,3 d and 7 d of medication. The histological slices of colon were observed by electron microscope after visual inspection. Results ： The body weights of rats were decreased after 3 days and recovered in 50 mg· kg-1 dose group and decreased in both 150 mg· kg-1 and 100mg· kg-1 dose groups after 7 days. The modeling time was not the main factor that affecting the damage degree of ulcerative colitis. In group 50 mg· kg-1, massive inflammatory ceils infiltrated in the colon and there were no pathology changes such as intestinal gland swelling or mucous cell abscission ,which accorded with pathological feature of ulcerative colitis. Conclusion：The establishment of ulcerative colitis model on rats only depends on the dose of TNBS but not modeling time within the 7 days and the model can be induced by 50 mg·kg-1 TNBS.